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176,133 results

Showing: 9241 - 9270 of 176133 results

  1. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  2. pLenti-H2B-iRFP720

    Plasmid
    #128961
    Purpose
    Lentiviral plasmid for expression of far red iRFP720 nuclear marker
    Depositor
    Carlos Carmona-Fontaine
    Insert
    Human histone H2B (H2BC21 Human)
    Use
    Lentiviral
    Tags
    iRFP720
    Expression
    Mammalian
    Promoter
    hPGK
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128961-plasmid-map-sequence-id-252385

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  4. OaAEP1_C247A

    Plasmid
    #200307
    Purpose
    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)
    Depositor
    Artur Krężel
    Article
    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27
    Insert
    Asparaginyl endopeptidase from Oldenlandia affinis
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Cysteine 247 to Alanine
    Promoter
    T7
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200307-plasmid-map-sequence-id-394865

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  6. pSFV3

    Plasmid
    #92072
    Purpose
    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Type
    Empty backbone
    Use
    Empty expression plasmid, for run-off transcripti…
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92072-plasmid-map-sequence-id-181621

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  13. Anti-GCN4 [IPI-C11L34] (Recombinant Antibody)

    Antibody
    #218104-rAb
    Purpose
    Anti-GCN4 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to HLENEVARLKK sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Article
    Zahnd et al J Biol Chem. 2004 Apr 30;279(18):18870-7
    Recommended Applications
    Western Blot
    Reactivity
    Budding yeast
    Source Species
    Rabbit
    Isotype
    IgG
    Trial Size
    Not available to purchase
    Available Since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  15. KRAB-dSpCas9

    Plasmid
    #205248
    Purpose
    In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    KRAB-dSpCas9
    Use
    CRISPR
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205248-plasmid-map-sequence-id-409108

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  22. pAAV-nEF-Con/Fon-ChR2-mCherry (AAV8)

    Viral Prep
    #137142-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-ChR2-mCherry (#137142). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-ChR2-mCherry plasmid DNA. nEF-driven, Cre and Flp-dependent expression of channelrhodopsin fused to mCherry for optogenetic activation.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mCherry (Cre and Flp-dependent)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  25. Pro-Code Vector Kit

    Plasmid Kit
    #1000000177
    Purpose
    Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Part of the Pro-Code Vector Kits.
    Depositor
    Brian Brown
    Application
    Barcoding, Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only

  27. pHR_TRE3G_mCherry_WPRE_PGK_tagBFP

    Plasmid
    #231952
    Purpose
    Lentiviral vector for inducible expression of the red fluorescent protein mCherry (TRE3G promoter) and constitutive expression of the blue fluorescent protein tagBFP (PGK promoter)
    Depositor
    Leonardo Morsut
    Insert
    mCherry
    Use
    Lentiviral
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    231952-plasmid-map-sequence-id-470261

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  28. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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Showing: 9241 - 9270 of 176133 results