167,318 results

1. pPB-cT3G-cERP2-FOS

   Plasmid

   #192924

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of FOS

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   FOS (FOS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-SopF

   Plasmid

   #137734

   Purpose

   Expresses EGFP fused to SopF

   Depositor

   Leigh Knodler

   Insert

   SopF
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCas9-T2A-GFP

   Plasmid

   #78548

   Purpose

   Cas9 protein with GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   In frame with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-PGK-hESRRB

   Plasmid

   #60434

   Purpose

   Over-expression of human ESRRB

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   ESRRB (ESRRB Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. 769 pME mCerulean-CAAX

   Plasmid

   #195957

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIX402 PHF2

   Plasmid

   #91700

   Purpose

   inducible lentiviral expression of PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-FOXM1

   Plasmid

   #153136

   Purpose

   Expression of Flag tagged human FOXM1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXM1 (FOXM1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn1-mTurquoise2

   Plasmid

   #99125

   Purpose

   An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mTurquoise2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTAGBFP2 Peroxisome visual barcode

   Plasmid

   #158668

   Purpose

   tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mTAGBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX iLOV

    Plasmid

    #26587

    Depositor

    John Christie

    Article

    Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

    Insert

    iLOV
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV Blast empty (w263-1)

    Plasmid

    #17486

    Purpose

    3rd gen lentiviral empty control vector, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKmK.T4

    Plasmid

    #125056

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 terminator
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-ESR1-Y537N

    Plasmid

    #116373

    Purpose

    Lentiviral expression of ESR1 Y537N

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Mutation

    Y537N

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO-EGFP

    Plasmid

    #50457

    Purpose

    Double floxed EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

    Plasmid

    #231591

    Purpose

    Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-2xALFATag-10xSunTag
    

    Expression

    Mammalian

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti.PGK.chFP.W

    Plasmid

    #51008

    Purpose

    Lentivector that expresses chFP from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    mChFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human PGK

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCG_IFITM1

    Plasmid

    #179955

    Purpose

    Expression vector for human IFITM1.

    Depositor

    Frank Kirchhoff, Konstantin Sparrer

    Article

    Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

    Insert

    IFITM1 (IFITM1 Human)
    

    Expression

    Mammalian

    Mutation

    P13A

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-S5E2-dTom-nlsdTom

    Plasmid

    #135630

    Purpose

    AAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    dTom-nlsdTom
    

    Use

    AAV

    Mutation

    None

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-hSyn1-eYFP (AAV Retrograde)

    Viral Prep

    #117382-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified pAAV-hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Promoter

    Syn

    Tags

    eYFP

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

27. pLentiRhoA2G

    Plasmid

    #40179

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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29. pENTR2b-mTurquoise2

    Plasmid

    #191014

    Purpose

    Gateway entry vector with the mTurquoise2 fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    mTurquoise2
    

    Use

    Gateway entry vector

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-TRIM28

    Plasmid

    #65397

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only