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Showing: 601 - 620 of 62130 results

  1. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid
    #136655
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136655-plasmid-map-sequence-id-273341

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  2. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid
    #136653
    Purpose
    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and hU6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136653-plasmid-map-sequence-id-273329

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  3. pGGA_RT_Sa_Cas9

    Plasmid
    #136962
    Purpose
    Goldengate/Greengate module for genome editing, using the SaCAS9.
    Depositor
    Klaus Theres
    Insert
    SaCAS9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    136962-plasmid-map-sequence-id-272380

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  4. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  6. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126880-plasmid-map-sequence-id-249730

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  7. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

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  8. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  12. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103856-plasmid-map-sequence-id-202299

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  14. HeFm1SpCas9

    Plasmid
    #92110
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92110-plasmid-map-sequence-id-177882

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  17. pCas59

    Plasmid
    #82396
    Purpose
    sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82396-plasmid-map-sequence-id-156156

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Showing: 601 - 620 of 62130 results