We narrowed to 3,337 results for: GCT

1. pQCXIX_sh_HOIP/RNF31_#6

   Plasmid

   #75252

   Purpose

   retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against human RNF31 (RNF31 )
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP773_pLenti-6xHRBKET-sgRNA

   Plasmid

   #211766

   Purpose

   Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pCMV and U6

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD5-U6-3-t-attP40

   Plasmid

   #133561

   Purpose

   gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   attP40 region guide RNAs
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-shRNA_Tet3

   Plasmid

   #85740

   Purpose

   shRNA against mouse Tet3

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   Tet3 (Tet3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATP1A1_G2_Dual_sgRNA

   Plasmid

   #173201

   Purpose

   Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330.Pten.b

   Plasmid

   #66588

   Purpose

   sgRNA for Pten deletion

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   Pten deletion (Pten Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

   Plasmid

   #101349

   Purpose

   2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta tat delta TAR delta env GFP in Nef Tet ope…

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. E42_gRunx1_gRNA1_dTet_NGFR

   Plasmid

   #189802

   Purpose

   Retroviral delivery of guide RNA against mouse Runx1

   Depositor

   Ellen Rothenberg

   Article

   Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   gRunx1_gRNA1 (Runx1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-C1-FAK-HA-V744G

   Plasmid

   #35040

   Depositor

   Anna Huttenlocher

   Article

   Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

   Insert

   focal adhesion kinase (Ptk2 Mouse)
   

   Use

   Tags

   2x HA and mCherry

   Expression

   Mammalian

   Mutation

   GFP-FAK-V744G was generated from GFP-FAK using th…

   Promoter

   Available since

   March 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMyc4ElbLuc.

    Plasmid

    #53246

    Purpose

    reporter plasmid containing 4 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc4ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSMP-SUZ12_1

    Plasmid

    #36390

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SUZ12 (SUZ12 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-EGFP-RIP1

    Plasmid

    #75163

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIPk1

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIPK1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSIL-eGFP-shACTN3

    Plasmid

    #52678

    Purpose

    shRNA against α-actinin-3 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN3 (Actn3 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMyc3ElbLuc

    Plasmid

    #53245

    Purpose

    reporter plasmid containing 3 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc3ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pUDE735

    Plasmid

    #103024

    Purpose

    expression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

    Plasmid

    #178100

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMyc1ElbLuc,

    Plasmid

    #53243

    Purpose

    reporter plasmid containing 1 myc cDNA binding sites

    Depositor

    Roger Davis

    Article

    Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

    Insert

    pMyc1ElbLuc (MYC Human)
    

    Use

    Tags

    Expression

    Mutation

    To prepare plasmids with one, two, three, and fou…

    Promoter

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CAG-eYFP-3x-miR708-5p-TS

    Plasmid

    #117381

    Purpose

    An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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