167,514 results

1. pCSC-NLS-EGFP

   Plasmid

   #221811

   Purpose

   Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)

   Depositor

   Baojin Ding

   Article

   Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

   Insert

   NLS-EGFP
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-p300 Core (Y1467F)

   Plasmid

   #61362

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-GAL4

   Plasmid

   #24344

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Expression

   Insect

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-MCS-TurboID-3xFLAG

   Plasmid

   #186814

   Purpose

   CAG promoter-driven expression of BioID2-fused protein (C terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Tags

   TurboID-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SpRYc-ABE8e-EGFP

   Plasmid

   #208340

   Purpose

   Expresses SpRYc-ABE8e in mammalian cells

   Depositor

   Pranam Chatterjee

   Article

   Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

   Insert

   ecTadA(8e)-nSpRYc
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Sc++ (1-1119), SpRY (1111-1368), D10A

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGT-S2

   Plasmid

   #122583

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. FSW-ALDH2-E504K-T2A-EGFP

   Plasmid

   #197037

   Purpose

   Express ALDH2 with E504K mutation from hSyn1 promoter, co-expressed with EGFP

   Depositor

   Ronald Hart

   Article

   Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

   Insert

   ALDH2-E504K
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E504K

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGT-Ah6

   Plasmid

   #122573

   Purpose

   MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   catP/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD4 galK-GT

    Plasmid

    #19183

    Depositor

    Al Fisher

    Article

    Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

    Insert

    galK (galK E.coli)
    

    Use

    Recombineering

    Tags

    50 nucleotides from N-term TAP, C-term TAP, and F…

    Available Since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR2.1-InC

    Plasmid

    #83959

    Purpose

    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

    Depositor

    Kai-Shun Chen

    Insert

    Internal Control sequence
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGT-B2

    Plasmid

    #122580

    Purpose

    MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-PDGFRb-HA

    Plasmid

    #204350

    Purpose

    Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    AAV

    Tags

    HA

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIC301

    Plasmid

    #69732

    Purpose

    Retroviral expression of Bub1

    Depositor

    Iain Cheeseman

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pGT-B3

    Plasmid

    #122581

    Purpose

    MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Hygro EYFP H148Q/I152L

    Plasmid

    #25874

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Available Since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJC175e-DddI

    Plasmid

    #179693

    Purpose

    Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pemiRFP670-N1

    Plasmid

    #136556

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSico-shNKCC1

    Plasmid

    #83027

    Purpose

    expresses an shRNA against NKCC1

    Depositor

    Angelique Bordey

    Article

    Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

    Insert

    shNKCC1
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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