169,099 results

1. pEGFP-NMHC II-C

   Plasmid

   #10843

   Depositor

   Robert Adelstein

   Article

   Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

   Insert

   NMHC II-C (Myh14 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-G-Creon shRNA[Control]

   Plasmid

   #181824

   Purpose

   This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

   Depositor

   Eun Mi Hwang

   Article

   Kim et al J Neurosci Methods. 2021 Dec 21:109452.

   Type

   Empty backbone

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   EF1a/U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pVDL9.3

   Plasmid

   #168299

   Purpose

   Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

   Depositor

   Víctor de Lorenzo

   Article

   Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

   Insert

   Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
   

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158653

   Purpose

   making transgenics expressing a UAS driven mCherry and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Mutation

   F70A/F108Y

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR P4-P1R-EGFP

   Plasmid

   #48348

   Purpose

   Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Mutation

   Contains a Kozak sequence. Does not contain a sto…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139095

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dCas12j3 KRAB

   Plasmid

   #188507

   Purpose

   Expresses FLAG tagged dCas12j3 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dCas12j2 VPR

   Plasmid

   #188518

   Purpose

   Expresses FLAG tagged dCas12j2 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D394A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Alpha 5 integrin-GFP

   Plasmid

   #15238

   Depositor

   Rick Horwitz

   Article

   Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

   Insert

   alpha 5 integrin (ITGA5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DsRed2-Mito-7

    Plasmid

    #55838

    Purpose

    Localization: Mitochondria, Excitation: 558, Emission: 583

    Depositor

    Michael Davidson

    Insert

    Mito (COX8A Human)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    KDEL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPSup

    Plasmid

    #149416

    Purpose

    Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

    Plasmid

    #241423

    Purpose

    Protein Transposition Cassette Containing CatN and CfaC

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG
    

    Tags

    His5, HA

    Expression

    Bacterial

    Available Since

    Oct. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS414-Syf1

    Plasmid

    #234114

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS414 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Expression

    Yeast

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+) Rheb(R15C)

    Plasmid

    #224290

    Purpose

    Cell transfection and expression of Rheb (R15C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rheb (RHEB Human)
    

    Expression

    Mammalian

    Mutation

    R15C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) Rheb(WT)

    Plasmid

    #224289

    Purpose

    Cell transfection and expression of Rheb

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rheb (RHEB Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge