163,297 results

1. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   Lambda-PPase
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFSp-GFP-YAP (5SA)

   Plasmid

   #174170

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   EFS

   Available since

   Sept. 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

   Plasmid

   #118593

   Purpose

   Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha/TRE3G

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (AAV PHP.eB)

   Viral Prep

   #214856-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) (#214856). In addition to the viral particles, you will also receive purified AiP14360: pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) plasmid DNA. minBG-driven expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Promoter

   Tags

   None

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-ExRai-AktAR2

   Plasmid

   #184047

   Purpose

   Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   ExRai-AktAR2
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-opto-hCaspase-8

   Plasmid

   #208786

   Purpose

   Inducibly expresses human Caspase 8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Caspase 8 (CASP8 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   CARD domain is removed

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I319V, N451D, D532N

   Promoter

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet-IRES-EGFP

   Plasmid

   #64238

   Purpose

   Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

   Depositor

   Maria Lung

   Article

   Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTet

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET.HaloTag.v7

   Plasmid

   #226197

   Purpose

   bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 hygro shRNA E-cadherin

    Plasmid

    #18802

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmGFP-ADAR1-p110

    Plasmid

    #117928

    Purpose

    ADAR1-p110 expression plasmid.

    Depositor

    Kumiko Ui-Tei

    Article

    Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

    Insert

    ADAR1-p110 (ADAR Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Use

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-neo-cterminal-3HA

    Plasmid

    #102643

    Purpose

    To generate three copies of the HA epitope tag on cDNAs.

    Depositor

    Michael E. Wright

    Type

    Empty backbone

    Use

    Tags

    3x HA (YPYDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmGFP-P2A-K(AAA)20-P2A-RFP

    Plasmid

    #105688

    Purpose

    Double fluorescent stalling reporter K(AAA)20

    Depositor

    Ramanujan Hegde

    Article

    Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

    Inserts

    P2A-3xFLAG-VHPbeta
    K(AAA)20
    P2A-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (from vector)

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SigmaR1-mNeonGreen

    Plasmid

    #226566

    Purpose

    Encodes SigmaR1 protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-mCh-Cry2WT

    Plasmid

    #101221

    Purpose

    Blue-light dependent optogenetic protein Cry2

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    Cry2PHR
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-FOSL1

    Plasmid

    #170687

    Purpose

    doxycycline-inducible overexpression of FOSL1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid

    #12252

    Purpose

    3rd generation lentiviral backbone

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge