164,890 results

1. pHBS838 TDP43 RRM-GFP

   Plasmid

   #98249

   Purpose

   TDP43 RRM-GFP reporter construct

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Internal GFP to replace RRM domains

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad33_ISTfu1_reRNA

   Plasmid

   #205355

   Purpose

   Bacterial expression of ISTfu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISTfu1 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST/LIfeAct-mCherry-N1

   Plasmid

   #40908

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   LifeAct
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   No stop codon to create c-terminal fusion

   Promoter

   CMV

   Available since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB426

   Plasmid

   #68691

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

   Plasmid

   #209283

   Purpose

   Expresses Voltron1 and jGCaMP7f in the zebrafish heart

   Depositor

   Adam Cohen

   Article

   Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

   Insert

   Voltron1_P2A_jGCaMP7f
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   zACNE20_myl7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK292 (mAID-mCherry2-NeoR)

   Plasmid

   #72830

   Purpose

   C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mCherry2-Neo
   

   Use

   CRISPR

   Tags

   mAID-mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF3117

   Plasmid

   #144593

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MZF1 (MZF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Wnt10A

   Plasmid

   #35920

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt10A (WNT10A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDN41

    Plasmid

    #61344

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS9 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TARDBP_Halo_N_allele

    Plasmid

    #178131

    Purpose

    Donor vector for endogenous tagging of human TARDBP at the N-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (TARDBP Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p405MET25

    Plasmid

    #15961

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIB2 gRNA (BRDN0001144754)

    Plasmid

    #75602

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIB2 gRNA (BRDN0001148141)

    Plasmid

    #75603

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.3/TO/V5-DEST-IGFBP2-Clover

    Plasmid

    #191008

    Purpose

    Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
    

    Use

    Lentiviral

    Tags

    Clover

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTA7001-DEST

    Plasmid

    #71745

    Purpose

    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GAL4

    Available since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM9121_His2-GFP11

    Plasmid

    #153511

    Purpose

    His2-GFP11 C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    His2-GFP11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6_(Gluc)_INT(GenPurpClon)

    Plasmid

    #68433

    Purpose

    General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct
    

    Use

    CRISPR; Cloning vector for generating int-like co…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

    Plasmid

    #71720

    Purpose

    Expressing an mRuby-tagged TIR1 in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
    

    Use

    Tags

    mRuby

    Expression

    Worm

    Mutation

    codon-optimized for expression in C. elegans; Two…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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