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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Sec61β

Plasmid

#246874

Purpose

Expression of mScarelt3-S2 fused with Sec61β. This construct is used for imaging the ER.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Sec61β

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (AAV1)

Viral Prep

#98927-AAV1

Purpose

Ready-to-use AAV1 particles produced from pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (#98927). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of GFPsm_myc spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Promoter

CAG

Tags

GFPsm-myc (spaghetti monster construct)

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only
pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV LIC cloning vector (1M)

Plasmid

#29656

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG- gate-pGK-HYG

Plasmid

#107397

Purpose

Gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

Plasmid

#194925

Purpose

mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

Depositor

Hao Xu

Article

Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

hPGK

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only
pHS18 (mANGPTL3-Strep)

Plasmid

#109111

Purpose

Expresses StrepII-tagged mouse ANGPTL3 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL3 (Angptl3 Mouse)

Tags

StrepII

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO V5 HSPH1

Plasmid

#19506

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 105kDa/110kDa protein 1 (HSPH1 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pHCMV_EEE_E1E2

Plasmid

#207272

Purpose

Mammalian expression of Eastern equine encephalitis virus E1E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

EEE_E1E2 (EEEVgp3 Eastern equine encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-BRCA1-mCherry

Plasmid

#195275

Purpose

express mCherry-BRCA1 fusion

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

mCherry-BRCA1

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-14-3-3ζ

Plasmid

#48798

Purpose

expresses human myc-14-3-3ζ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAZ (YWHAZ Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHJ322

Plasmid

#20677

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 558 to Asp

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-Ascl1-puro

Plasmid

#97329

Purpose

Generating lentiviruses to express Ascl1 and puromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Promoter

TET-ON

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pC3.1_CMV_oROS-G

Plasmid

#216111

Purpose

Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-G

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-165

Plasmid

#118545

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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