We narrowed to 8,464 results for: gnal

1. pLenti-Cas9-NS-A

   Plasmid

   #207823

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-ZNF746-C571A

   Plasmid

   #207826

   Purpose

   Dox-inducible expression of ZNF746-C571A

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746-C571A (ZNF746 Human)
   

   Use

   Lentiviral

   Mutation

   C571A

   Promoter

   TRE

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-ZNF746-V5-miniTurbo

   Plasmid

   #207820

   Purpose

   C-terminal tagged ZNF746 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746-V5-miniTurbo (ZNF746 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Promoter

   UBC

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEos3.2trLATo

   Plasmid

   #203746

   Purpose

   Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLATo (Lat Mouse)
   

   Tags

   ER translocation signal - mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

   Plasmid

   #192452

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Tags

   3x Nuclear localization signal (NLS) and mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LIC-Z-Y-L

   Plasmid

   #153546

   Purpose

   this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Expression

   Mammalian

   Mutation

   with 6 Y in 3 ITAMs mutated to L

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFFΔC-EGFP

   Plasmid

   #86059

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-cystin-CTS

   Plasmid

   #86066

   Purpose

   A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Cystin (Cys1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 28-35

   Promoter

   tac

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CFFΔC-Myc

    Plasmid

    #86060

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    contains CD8a luminal and transmembrane domains a…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3 T7 Jip1(1-470)

    Plasmid

    #51724

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 471-660

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 T7 Jip1(471-660)

    Plasmid

    #51766

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 1-470

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcdna3 T7Jip1(1-126)

    Plasmid

    #51702

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 127-660

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 T7 Jip1(1-281)

    Plasmid

    #51700

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 282-660

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCCLc-MND-A0201-SABR-Backbone

    Plasmid

    #119050

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mito-SBFP2

    Plasmid

    #187964

    Purpose

    Mammalian cell expression of a fluorescent SBFP2-tagged mitochondrial marker

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mito (COX8A Human)
    

    Tags

    SBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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