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We narrowed to 6,854 results for: itch

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Showing: 6481 - 6500 of 6854 results

  2. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

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  5. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid
    #22017
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutation T17N.
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22017-plasmid-map-sequence-id-138512

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  8. qTAG-N-Blast-3xHA-LMNB1

    Plasmid
    #207778
    Purpose
    Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-3xHA Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207778-plasmid-map-sequence-id-416192

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  9. pCS2+-Y276H PHYB-CAAX

    Plasmid
    #154911
    Purpose
    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…
    Promoter
    CMV
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154911-plasmid-map-sequence-id-308717

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  11. qTAG-C-Solo-mStayGold-CLTC

    Plasmid
    #227313
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CLTC Homology Arms flanking a mStayGold Tag (CLTC Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227313-plasmid-map-sequence-id-451694

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  12. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid
    #174631
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174631-plasmid-map-sequence-id-346982

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  14. pCIBN-hTRF2-tagRFP-T

    Plasmid
    #103812
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103812-plasmid-map-sequence-id-199277

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  15. pROF346

    Plasmid
    #155331
    Purpose
    Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155331-plasmid-map-sequence-id-311105

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  16. qTAG-N-Blast-ultraID-LMNB1

    Plasmid
    #207775
    Purpose
    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207775-plasmid-map-sequence-id-416196

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  17. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid
    #174633
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174633-plasmid-map-sequence-id-346989

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Showing: 6481 - 6500 of 6854 results