We narrowed to 1,915 results for: MB;
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Plasmid#91810PurposeYeast expression of S. cerevisiae Rpb1 with a P1494A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged proline 1494 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3508 (YCp LEU2 Rpb1 Y1473A)
Plasmid#91811PurposeYeast expression of S. cerevisiae Rpb1 with a Y1473A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged tyrosine 1473 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3509 (YCp LEU2 Rpb1 T1471A)
Plasmid#91812PurposeYeast expression of S. cerevisiae Rpb1 with a T1471A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged threonine 1471 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3511 (YCp LEU2 Rpb1 P1472A)
Plasmid#91813PurposeYeast expression of S. cerevisiae Rpb1 with a P1472A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged proline 1472 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3566 (pDEST15_Spt6 1223-1451)
Plasmid#91814PurposeBacterial expression of residues 1223-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
TagsGSTExpressionBacterialMutationdeleted residues 1-1222PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH1141 (pET151_Spt6 1247-1451)
Plasmid#91815PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6DepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationdeleted residues 1-1246PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJPL3[flp-6p::cmk-1::GFP]
Plasmid#124613PurposeUsed to confirm the expression of the trans-spliced GFP reporter in the expected ASE neuronsDepositorInsert[flp-6p::cmk-1::GFP]
ExpressionWormAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC681
Plasmid#107019PurposeGFP reporter of lag-2 driven by 405 bp of promoterDepositorInsertGFP-PH
TagsGFPExpressionWormPromoterlag-2Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGC643
Plasmid#107016PurposeGFP reporter of lag-2 driven by 0.5 kb promoterDepositorInsertGFP-PH
TagsGFPExpressionWormPromoterlag-2Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS345
Plasmid#89349PurposeBeetle Luciferase C-term tagging vectorDepositorInsertBeetle luciferase
UseC-terminal tagging vectorAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS346
Plasmid#89350PurposeRenilla Luciferase C-term tagging vectorDepositorInsertRenilla luciferase
UseC-terminal tagging vectorAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
PromoternoneAvailable SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-CALB2-3'UTR delta ARE&mir30
Plasmid#74430PurposeCalb2 UTR with mutated AU binding sequence and mir-30 site 1 and 2DepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-WRAP53beta-siRNA-resistant
Plasmid#64680Purposeexpresses siRNA resistant WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
TagsFLAGExpressionMammalianMutationsiRNA resistantPromoterCMVAvailable SinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
NC11 pCDNA3.1 VENUS WTX K292N
Plasmid#36966DepositorAvailable SinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
NC8 pCDNA3.1 VENUS WTX S286D
Plasmid#36963DepositorAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
NC7 pCDNA3.1 VENUS WTX S286A
Plasmid#36962DepositorAvailable SinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
NC10 pCDNA3.1 VENUS WTX T289A
Plasmid#36965DepositorAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
NC9 pCDNA3.1 VENUS WTX S286E
Plasmid#36964DepositorAvailable SinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro dn-cHSF1
Plasmid#134732PurposeLentiviral expression vector for constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseLentiviralExpressionMammalianMutationDeletion of amino acids 186-202, 379−529PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MIGR1-Cdc42-1
Plasmid#179517PurposeRetroviral expression of Cdc42 splice variant 1DepositorAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000231737
Plasmid#78162PurposeLuciferase controlDepositorInsertLuciferase
UseRNAiPromoterhU6 and hU6Available SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000297275
Plasmid#78152PurposeshCDK4 targeting 3UTRDepositorAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3E-hGPR56 e1m promoter normal
Plasmid#52298PurposeReporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.DepositorInsertADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
UseLuciferaseAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI
Plasmid#196223PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI anchor, IgK-chain, and Myc epi tagExpressionMammalianAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)-mNeonGreen
Plasmid#205021PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorT-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-GCaMP
Plasmid#154082PurposeIndependent expression of QuasAr and GCaMP under human synapsin 1 promoterDepositorInsertQuasAr-P2A-GCaMP6f
UseAAVExpressionMammalianMutationChanged Tyrosine 327 to AlaninePromoterhuman synapsin 1Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mOrange-P2A-hKvb1
Plasmid#154084PurposeIndependent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoterDepositorInsertmOrange-P2A-human Kvb1 (KCNAB1 Human, Synthetic)
UseAAVExpressionMammalianPromoterhuman synapsin 1Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shNts
Plasmid#132717PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensinDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
PromoterPEZ3Available SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC403 SMARCB1 var 2
Plasmid#111185PurposeInducible vector (V5 tagged) with SMARCB1 variant 2DepositorAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTol2-UAS-zArchon2-KGC-EGFP-ER2
Plasmid#108428PurposeCodon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmidDepositorInsertzArchon2-KGC-EGFP-ER2
UseZebrafishPromoterUASAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-Vimentin-17-C
Plasmid#108414PurposeVimentin fused to monomeric near-infrared fluorescent protein miRFPDepositorAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1-sgAAVS1
Plasmid#83906Purposestable knockoutDepositorInsertadeno-associated virus integration site 1 (AAVS1 Human)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR
Plasmid#196219PurposeFluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFRDepositorHas ServiceAAV2InsertpAAV CAG FLEX iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ
Plasmid#196224PurposeFluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE
Plasmid#192202PurposeRetro-HER2CAR-PRODH2DepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
TagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable SinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DN-RFX
Plasmid#52296PurposeDominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.DepositorInsertDominant negative RFX
TagsMycExpressionMammalianPromoterCAG promoterAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-puro_V5-LOV-Tb-Miro1v4
Plasmid#253796PurposeExpress V5-LOV-Turbo2-myc-Miro1-v4 (peroxisomal varient) under TRE3G (tetracycline inducible) promoterDepositorInsertV5-LOV-Turbo2-myc-Miro1-v4 (RHOT1 Human)
UseLentiviral; Tetracycline inducible promoterExpressionMammalianPromoterpTRE_tetracycline inducibleAvailable SinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-puro_V5-LOV-Tb-myc-MIRO1-TM-KQR (OMM)
Plasmid#253797PurposeExpress V5-LOV-Turbo2-myc-Miro1-TM-KQR (OMM) under TRE3G (tetracycline inducible) promoterDepositorInsertV5-LOV-Tb-myc-Miro1-TM-KQR (OMM) (RHOT1 Human)
UseLentiviral; Tetracycline inducible promoterExpressionMammalianPromoterpTRE_tetracycline inducibleAvailable SinceApril 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEt28a-SilkTag-CBM-H6
Plasmid#230903PurposeBacterial expression of SilkTagDepositorInsertSilkTag-CBM-H6
TagsHisTagExpressionBacterialAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEt28a-H6-SUMO-SilkCatcher
Plasmid#230902PurposeBacterial expression of SilkCatcherDepositorInsertH6-SUMO-SilkCatcher
TagsHisTag, thrombin, SUMOExpressionBacterialAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV R354H SPOP 28-359
Plasmid#197011PurposeE. coli expression vectorDepositorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
Plasmid#91819PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
Plasmid#91820PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3567 (pET151_Spt6 1247-1451 R1282H)
Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
Tags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only