We narrowed to 30,902 results for: REP

1. Strep-TGT-His

   Plasmid

   #206495

   Purpose

   Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

   Insert

   E. coli tRNA Guanine Transglycosylase
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Annexin A7-TEV-TwinStrep

   Plasmid

   #198635

   Purpose

   Annexin A7 fibrillization and lipid binding

   Depositor

   Lalit Deshmukh

   Article

   Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

   Insert

   Annexin A7 (ANXA7 Human)
   

   Use

   Tags

   TEV-GS-Dual Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFsynW SYT1deltaC2AB reporter

   Plasmid

   #197279

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HyWnt3-ΔRep-D3:Luc

   Plasmid

   #192990

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D3)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D3

   Promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HyWnt3-ΔRep-D1:Luc

   Plasmid

   #192988

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D1

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HyWnt3-ΔRep-D2:Luc

   Plasmid

   #192989

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D2

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HyWnt3-ΔRep-D4:Luc

   Plasmid

   #192991

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D4

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACE-K2P4.1-StrepTagII

   Plasmid

   #191474

   Purpose

   Expresses K2P4.1 with C-terminal StrepTagII

   Depositor

   Arthur Laganowsky

   Article

   Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

   Insert

   K2P4.1 (KCNK4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   N104Q N108Q

   Promoter

   polH

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

   Plasmid

   #185299

   Purpose

   Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   pvLEA22mer_shuffle_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid

    #185966

    Purpose

    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid

    #185965

    Purpose

    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid

    #185964

    Purpose

    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid

    #185963

    Purpose

    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    EGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

    Plasmid

    #185317

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

    Plasmid

    #185313

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid

    #185302

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

    Plasmid

    #180314

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 )
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    SEPT9_i1-i5

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnCS_mEGFP-DSep2_Peanut-Strep

    Plasmid

    #174496

    Purpose

    bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    Strep and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pnCS_DSep2_Peanut-TEV-Strep

    Plasmid

    #174495

    Purpose

    bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACEBac1-Q22YU3-2xStrep

    Plasmid

    #170315

    Purpose

    Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag II

    Depositor

    Andrew Carter

    Article

    Mali et al Science. 2021 Feb 26;371(6532):910-916.

    Insert

    Q22YU3 (TTHERM_00122270 Tetrahymena thermophila)
    

    Use

    Tags

    Strep-Tag II

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge