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Showing: 641 - 660 of 26148 results

  4. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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  5. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  6. qTAG-N-Solo-sTagRFP-TUBA1B

    Plasmid
    #207765
    Purpose
    Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207765-plasmid-map-sequence-id-413791

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  13. ystagRFP-T2A-mTq2 pdrf1-GW

    Plasmid
    #168072
    Purpose
    Produces equimolar expression of ystagRFP and mCherry for use in yeast
    Depositor
    Bas Teusink
    Insert
    ystagRFP-T2A-mTq2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168072-plasmid-map-sequence-id-332113

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  15. pENTR-L4R1-hCMVstar1+bgloInt

    Plasmid
    #139528
    Purpose
    Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    hCMVstar1 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139528-plasmid-map-sequence-id-274681

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  16. pTH825-CEN-minRLuc/slowstaCFLuc

    Plasmid
    #115370
    Purpose
    Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Gcn4 5'-UTR + Firefly Luciferase
    Renilla luciferase (codon minimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the least favour…
    Promoter
    ADH1 and TDH3
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115370-plasmid-map-sequence-id-238823

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  18. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

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Showing: 641 - 660 of 26148 results