We narrowed to 7,126 results for: poly

Showing: 6701 - 6720 of 7126 results

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pAG416GAL10-PV_3C

Plasmid

#203472

Purpose

Expresses PV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

PV 3C protease (PVgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCB’ CsoS2 ΔNTD

Plasmid

#162705

Purpose

Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

Deletion of residues 2-256 of CsoS2, Y131D

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K63

Plasmid

#17606

Purpose

Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K63 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TAP DHX36

Plasmid

#53547

Purpose

mammalian expression of DHX36

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

DHX36 (DHX36 Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48

Plasmid

#17605

Purpose

Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48 only, other lysines mutated to arginines. Enh…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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GIPR-DuET

Plasmid

#213252

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GIPR (GIPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Endo-INPP5E

Plasmid

#236005

Purpose

Endosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Endo-INPP5E (INPP5E Synthetic, Human)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Ratiometric FPX sensor for ERK kinase activity

Plasmid

#60974

Purpose

For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A-WW domain-ERK substrate-ddFP B

Tags

ERK substrate is before ddFP copy B, WW domain is…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pHIV-EGFP.Flag-EZH2

Plasmid

#220243

Purpose

Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway Cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.MTF2

Plasmid

#125169

Purpose

Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

MTF2 (MTF2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Tags

His

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available Since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-FLTID-GSQ-RBXN-P2A-BLAST

Plasmid

#208041

Purpose

Enables constitutive expression of TurboID alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

TurboID

Use

Lentiviral

Promoter

EFS

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TRC.mKO2_shGFP

Plasmid

#110318

Purpose

Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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