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Plasmid#19717DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationNOTE: long splice variant with 3bp deletion and 1…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_59.F2
Plasmid#60207PurposeExpresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
UseTagsFKBP12 and Nuclear localisation signalExpressionMammalianMutationamino acids 19-59 of the humanized version of Cre…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorInsertTCR beta (Tcrb Mouse)
UseMouse TargetingTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-TTF-1-HDD
Plasmid#74991PurposeRetroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deletedDepositorInsertTTF-1 HDD mutant (NKX2-1 Human)
UseRetroviralTagsNoneExpressionMammalianMutationHDD = homeodomain deletedPromoterAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLK3 1-334-pEGFP
Plasmid#23266DepositorInsertPLK3 (PLK3 Human)
UseTagspEGFPExpressionMammalianMutationaa 1-334PromoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA (short) wt
Plasmid#19723DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationNOTE: short splice variant Long Sp. Variant: MV…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAimR
Plasmid#98687PurposeExpresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tagDepositorInsertAimR
UseTagsHis-tagExpressionBacterialMutationPromoterNativeAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GTU-g
Plasmid#81094PurposeGene Tagging vector URA/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTW080
Plasmid#115928PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/30 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-TdT-guide1-PGK-Puro
Plasmid#102903PurposeEBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.DepositorInsertTdT-guide1-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE97 mFZ8-CS2+
Plasmid#16800DepositorInsertmFZ8-CS2+ (Fzd8 Mouse)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-19-G
Plasmid#124676PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-iRPT
Plasmid#140763PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassetteDepositorInsertsiRFP713
PuroR
rtTAM2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW088
Plasmid#115936Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 3'UTR No E1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW094
Plasmid#115937Purpose3'UTR insert for replicon MoCloDepositorInsertLvl0 E1-3'UTR End
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y115S/Y132S/Y139S
Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q443P
Plasmid#216402PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 443 to Proli…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y450S/Y452S/Y456S
Plasmid#216410PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutationsDepositorInsertEfg1-ΔDBD-Y450S/Y452S/Y456S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 450, 452 and 456 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y478S/Y482S/Y484S
Plasmid#216411PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutationsDepositorInsertEfg1-ΔDBD-Y478S/Y482S/Y484S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 478, 482 and 484 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
UseTags6xHis and CUbo-TwinStrepExpressionBacterialMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-His6
Plasmid#212780PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP
Plasmid#212769PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-VSV
Plasmid#212752PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(63)-VSV
Plasmid#212753PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(48)-VSV
Plasmid#212744PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-VSV
Plasmid#212743PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(63)-FLAG
Plasmid#212808PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cellsDepositorInsertE3(63)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-FLAG
Plasmid#212797PurposeConstitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cellsDepositorInsertNUbo
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo(K48R/K63R)-yeGFP
Plasmid#212774PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-yeGFP-His6
Plasmid#212783PurposeC-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-yeGFP-His6
Plasmid#212784PurposeC-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-yeGFP-His6
Plasmid#212785PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R/K63R)-9myc
Plasmid#212789PurposeC-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only