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Showing: 1321 - 1360 of 8498 results

  6. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  9. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid
    #138524
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    PRC2-binding G-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138524-plasmid-map-sequence-id-292291

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  12. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

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  33. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

    Plasmid
    #209783
    Purpose
    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Article
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    miR122 ts
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    cTNT
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209783-plasmid-map-sequence-id-415445

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Showing: 1321 - 1360 of 8498 results