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Showing: 1651 - 1700 of 2660 results

  13. GFP Cav2.2 W391A pRK5

    Plasmid
    #206084
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding
    Depositor
    Annette Dolphin
    Article
    Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    206084-plasmid-map-sequence-id-412224

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  18. pcDNA3-EngPDm

    Plasmid
    #64040
    Purpose
    Mammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    Pax8 paired domain (Pax8 Mouse)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    paired domain, aa 1-147, with aa 30 to 32 changed…
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3-EngHDm

    Plasmid
    #64038
    Purpose
    Mammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2
    Insert
    TTF-1 (TTF1 dog)
    Use
    Tags
    Eng (N-terminal repressor domain of the drosophi…
    Expression
    Mammalian
    Mutation
    homeodomain, aa 154-226, with aa 48–50 replaced b…
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. Reduced Expression Alpha-5-Integrin-GFP

    Plasmid
    #30450
    Depositor
    Rick Horwitz
    Insert
    alpha 5 integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    91-544 of enhancer region of CMV promoter removed…
    Promoter
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  23. pcDNA3.1/hpa5

    Plasmid
    #59972
    Purpose
    integrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)
    Depositor
    Timothy Springer
    Insert
    human integrin a5 headpiece (ITGA5 Human)
    Use
    Tags
    acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pDEST26-NAT1-C-HA

    Plasmid
    #195178
    Purpose
    Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Stephanie Roessler
    Insert
    NAT1 (NAT1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    195178-plasmid-map-sequence-id-423104

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  36. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69024-plasmid-map-sequence-id-127982

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  37. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid
    #221566
    Purpose
    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 16nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221566-plasmid-map-sequence-id-437904

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  38. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

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  39. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

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  41. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Use
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  42. pCiGSΔΨ

    Plasmid
    #108312
    Purpose
    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    David Russell
    Article
    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.
    Insert
    gag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    pCiGSΔΨ is a newer version of the pCiGS Gag expre…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108312-plasmid-map-sequence-id-211440

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  44. Human a4 nicotinic subunit crystallization construct

    Plasmid
    #85841
    Purpose
    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85841-plasmid-map-sequence-id-171121

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  45. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Article
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119164-plasmid-map-sequence-id-232464

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  46. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

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  50. Cx26-msfGFP

    Plasmid
    #69016
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx26 (Gjb2 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69016-plasmid-map-sequence-id-124712

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Showing: 1651 - 1700 of 2660 results