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Halo-SspB-PPM1H

Plasmid

#232870

Purpose

Expresses SspB-PPM1H in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

Halo-SspB-PPM1H (PPM1H )

Tags

Halo-SspB

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ spike_del19

Plasmid

#155297

Purpose

Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

Depositor

Raffaele De Francesco

Article

Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

Insert

SARS-Cov2 spike_deleted (S SARS-Cov2)

Use

Generation of sars-cov-2-pseudotyped lentiviral p…

Mutation

Deletion in C-term 19 aa of spike

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Promoter

1700bp upstream of BLBP gene

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469V

Plasmid

#131718

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469V

Promoter

CMV

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Tags

mRFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

aa2-439

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-puro/RAF1

Plasmid

#131725

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HA-mDyn1 pcDNA3

Plasmid

#36263

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 1 (Dnm1 Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only
pLPC-MYC-hTRF1

Plasmid

#64164

Purpose

Retroviral vector expressing human TRF1 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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VPS13B^GFP

Plasmid

#224588

Purpose

codon optimized GFP tagged VPS13B for expression in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

VPS13B^GFP (VPS13B Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

Plasmid

#175549

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

WAPL (Wapl Mouse)

Use

Mouse Targeting

Tags

AID[71-114]-eGFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

aa2-500, inserted alanin at aa434

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBS xChordin

Plasmid

#24975

Depositor

Edward De Robertis

Article

Sasai et al Cell. 1994 Dec 2. 79(5):779-90.

Insert

Chordin (chrd.1.S Frog)

Expression

Bacterial

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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LV-LIN28A

Plasmid

#117057

Purpose

Overexpression of mouse LIN28A

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

LIN28A (Lin28a Mouse)

Use

Lentiviral

Promoter

hPGK promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR_Hu_BIRC5(WT)

Plasmid

#136351

Purpose

Human BIRC5 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

BIRC5 (BIRC5 Human)

Use

Gateway entry vector

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA421-Cas9tr

Plasmid

#138709

Purpose

Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

tracrRNA-Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Article

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Insert

Dynamin 2 K44A (Dnm2 Rat)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus Frzb-1

Plasmid

#24694

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

Frzb-1 (frzb-1 Frog)

Expression

Mammalian

Available Since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
alpha1-EGFP

Plasmid

#118952

Purpose

GABAA receptor expression (alpha1 subunit). EGFP tag at the C-terminus.

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRA1 (Gabra1 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sFRP2

Plasmid

#24696

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

secreted frizzled-related sequence protein 2 (sfrp2 Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
HA-mDyn2 pcDNA3

Plasmid

#36264

Depositor

Pietro De Camilli

Article

Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

Insert

Dynamin 2 (Dnm2 Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only
pCD5-PR8-TEV-sfGFP-twinStrep

Plasmid

#157933

Purpose

Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

Insert

A/PuertoRico/8/34 hemagglutinin (HA Synthetic)

Tags

sfGFP & TwinStrep

Expression

Mammalian

Mutation

E190D

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
xGoosecoid

Plasmid

#24977

Depositor

Edward De Robertis

Article

Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

Insert

Goosecoid (gsc-a Frog)

Expression

Bacterial

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 GSK3-GFP

Plasmid

#29680

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

glycogen synthase kinase 3

Tags

GFP

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-NMYC TRF2 deltaB deltaM

Plasmid

#16069

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPC-N FLAG

Plasmid

#12521

Depositor

Titia de Lange

Insert

N FLAG

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Septin 11 isoform II

Plasmid

#120318

Purpose

Septin 11 isoform II expression (mCherry-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Hygro-NMYC TRF2 deltaB deltaM

Plasmid

#16355

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pLPC-N MYC

Plasmid

#12540

Depositor

Titia de Lange

Insert

N MYC

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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