We narrowed to 2,423 results for: LRE

1. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST40-2XFL-Src-S4-S32-KD

   Plasmid

   #140299

   Purpose

   Expresses Flag-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   lacks the UD region

   Promoter

   CMV

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST40-2XFL-Src-S32-KD

   Plasmid

   #140311

   Purpose

   Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Contains only the SH3, SH2, and KD regions

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR LAT-FFF/Zap70 iLID-Drop

   Plasmid

   #171032

   Purpose

   Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PXPR007 sgCIC-2

   Plasmid

   #74959

   Purpose

   Cas9 + sgCIC-2 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   CIC (CIC Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 CDK9 (D167N) HA (P#811)

   Plasmid

   #14641

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant. Please see deposit…

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEX-SaCas9-U6-sgGria2

   Plasmid

   #124854

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-CMV-mouse Gfi1-GFP

   Plasmid

   #91892

   Purpose

   3rd generation lentiviral expression of mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Gfi1 (Gfi1 Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Unknown

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PXPR007 sgCIC-1

    Plasmid

    #74953

    Purpose

    Cas9 + sgCIC-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTet-O-E2F7-T2A-PuroR

    Plasmid

    #162348

    Purpose

    Lentiviral expression of E2F7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV pCAG-mRuby3-WPRE

    Plasmid

    #107744

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Article

    Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HER3-DMVF-181-R

    Plasmid

    #87621

    Purpose

    C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    HER3-DMVF181-C (ERBB3 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-FLEXfrt-SaCas9-U6-sgGria1

    Plasmid

    #124867

    Purpose

    Mutagenesis of Gria1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria1 (Gria1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-WT-ATPIF1

    Plasmid

    #85403

    Purpose

    Retroviral vector expressing wild type human ATPIF1

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6,CBh

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LV-hHOXA13

    Plasmid

    #135561

    Purpose

    Express hHOXA13 and EGFP in mammalian cells

    Depositor

    Jose Teixeira

    Article

    George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

    Insert

    Homeobox A13 (HOXA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1 shETV5-B

    Plasmid

    #74978

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcmv3-STING-C1471S-FLAG

    Plasmid

    #198188

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C1471S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 147 mutates to …

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid

    #154775

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in GFP reporter, UUA anticodon and G10A, …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.5 shETV5-A

    Plasmid

    #74977

    Purpose

    shETV5 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV5 (ETV5 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139087

    Purpose

    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

    Plasmid

    #154265

    Purpose

    Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    FLAG-CRTC1/MAML2 fusion oncogene
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-Bmpr2ΔKinase gRNA resistant

    Plasmid

    #163411

    Purpose

    Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted. gRNA-t…

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-tetO-HA-GFI1B

    Plasmid

    #125599

    Purpose

    doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    Growth Factor Independent 1B transcriptional repressor (GFI1B Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1 shETV4-A

    Plasmid

    #74975

    Purpose

    shETV4 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO.5 shETV4-B

    Plasmid

    #74976

    Purpose

    shETV4 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcmv3-STING-C64S,C65S-FLAG

    Plasmid

    #198186

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 64/65 mutates t…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAVS1-Puro CAG IL13-CAR

    Plasmid

    #157742

    Purpose

    knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Chimeric antigen receptor targeting IL13
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTet-O-KLF7-T2A-PuroR

    Plasmid

    #162346

    Purpose

    Lentiviral expression of KLF7 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXPR_BRD003 sgCIC-1

    Plasmid

    #74966

    Purpose

    sgCIC-1, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

    Plasmid

    #154776

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in GFP reporter, hybrid PylT with A…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTet-O-NEUROG3-T2A-PuroR

    Plasmid

    #162341

    Purpose

    Lentiviral expression of NEUROG3 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    NEUROG3 (NEUROG3 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid

    #164902

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T17N mutation in the Rac1 domain which make the s…

    Promoter

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-FLEX-SaCas9-U6-sgGria3

    Plasmid

    #124855

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

    Plasmid

    #124865

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAS_4xhyb PylT A41AA C55A sfGFP 150TAG

    Plasmid

    #154772

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, hybrid PylT with A41AA an…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

44. pAG416GAL-ccdB-mRuby3

    Plasmid

    #166139

    Purpose

    Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pNL-CEF-IgA1

    Plasmid

    #187009

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80066

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80067

    Purpose

    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

    Plasmid

    #154777

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAA 150TAG in GFP reporter, hybrid PylT with …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. PXPR007 sgATXN1L-1

    Plasmid

    #74962

    Purpose

    Cas9 + sgATXN1L-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. PXPR007 sgATXN1L-2

    Plasmid

    #74963

    Purpose

    Cas9 + sgATXN1L-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge