We narrowed to 150,751 results for: ins

1. pLX_TRC311 ETV1

   Plasmid

   #74981

   Purpose

   Constitutive ETV1 ORF, blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV1 (ETV1 Human)
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD-MPR-GFP-RUSH

   Plasmid

   #202797

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   M6PR (M6PR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed 40 K (AAA) with R (AGA)

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 TAP DHX36

   Plasmid

   #53547

   Purpose

   mammalian expression of DHX36

   Depositor

   Kathleen Collins

   Article

   Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   TAP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTarget

   Plasmid

   #214052

   Purpose

   Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Target sequence
   

   Use

   Target rna plasmid

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHochTypeIII

   Plasmid

   #214039

   Purpose

   Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Cas10
   

   Use

   CRISPR

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATG-2460

   Plasmid

   #108713

   Purpose

   EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt-copGFP fusion
   

   Use

   Lentiviral

   Tags

   CBR2opt fused to copGFP

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   elongation factor-1

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Has Service

   AAV1

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX Emt-SH2

   Plasmid

   #46424

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Emt (ITK Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 223-346

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCST-AvicFP4

    Plasmid

    #129507

    Purpose

    Expresses AvicFP4 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AvicFP4
    

    Expression

    Bacterial

    Mutation

    One of two variants of AvicFP4 tested with essent…

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP-PRM-5R

    Plasmid

    #112155

    Purpose

    Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of Abl1

    Promoter

    CMV

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK 3xFLAG-MAVS hygro

    Plasmid

    #158634

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DRH313: FCK-CheRiff-eGFP

    Plasmid

    #51693

    Purpose

    Channelrhodopsin with improved sensitivity and minimal red light photocurrent

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    CheRiff-eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-PA-Rac1

    Plasmid

    #22024

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+) NRP1 b1 (273-427)

    Plasmid

    #162734

    Purpose

    Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1 domain residues 273-427
    

    Expression

    Bacterial

    Mutation

    Codon optimised

    Available Since

    April 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGEX Sap-SH2

    Plasmid

    #46477

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sap (SH2D1A Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 4-128

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-TEV-CyOFP1

    Plasmid

    #105799

    Purpose

    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-CyOFP1

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIDK-HDAC1fl

    Plasmid

    #109158

    Purpose

    Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-cFos-XRI-V5

    Plasmid

    #178058

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-V5
    

    Use

    AAV

    Tags

    V5-MBP

    Expression

    Mammalian

    Promoter

    Mouse c-fos promoter

    Available Since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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