We narrowed to 18,595 results for: MUT

1. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRK5-HA-Ubiquitin-K6

   Plasmid

   #121151

   Purpose

   Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Expression

   Mammalian

   Mutation

   All Ks apart from K6 mutated to Rs

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. huCaV2.2 (pSAD442-1)

   Plasmid

   #62574

   Purpose

   Human CaV2.2 expression in mammalian cells

   Depositor

   Diane Lipscombe

   Article

   Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

   Insert

   CACNA1B (CACNA1B Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDG462

   Plasmid

   #100903

   Purpose

   SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171594

   Purpose

   Strong human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA6.2EmGFP-DAXX

   Plasmid

   #169726

   Purpose

   Mammalian expression construct of GFP tagged full length DAXX

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX (DAXX Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.0-TERT WT

   Plasmid

   #84924

   Purpose

   luciferase reporter for TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   TERT

   Available Since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. iBE4max YE1 NG CLYBL

   Plasmid

   #174570

   Purpose

   doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Expression

   Mammalian

   Promoter

   tet ON

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Tau (wt) -VC

    Plasmid

    #87369

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pInducer20 TIFA T9A

    Plasmid

    #231590

    Purpose

    TIFA gene mutated in position T9A (mutation on phosphorylation site) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    TIFA (TIFA Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    T9A

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSH-CAG-AtAFB2.F74A-IRES-puro

    Plasmid

    #216245

    Purpose

    Express AtAFB2.F74A mutant without tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIB-HIS-TEV-CKS1

    Plasmid

    #177013

    Purpose

    Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CKS1 (CKS1B Synthetic, Human)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pF145 pAcGFP1 SOD1G93A

    Plasmid

    #26406

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G93A

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP KNL1 wt hardened and RVSF->AAAA

    Plasmid

    #45225

    Depositor

    Iain Cheeseman, Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)
    

    Mutation

    RVSF mutated to AAAA

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-GFP/CALR ins5

    Plasmid

    #214701

    Purpose

    Mammalian expression of human CALR ins5

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR ins5 (CALR Human)
    

    Use

    Retroviral

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3xFLAG CMV14 / BRAF

    Plasmid

    #131710

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VN-Tau (wt)

    Plasmid

    #87368

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge