Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,887 results for: Coli

Showing: 7101 - 7120 of 9887 results

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSTTa-GNAI1

Plasmid

#248651

Purpose

Vector for protein expression of 2x StrepII-tagged human heterotrimeric G protein GNAI1 in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GNAI1 (GNAI1 Human)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pHS1623

Plasmid

#239833

Purpose

WT OrufIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFMtail

Plasmid

#232056

Purpose

Expresses construct for surface tethering: R. norvegicus NFM tail domain

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Medium tail domain

Tags

6xHis, thrombin

Expression

Bacterial

Mutation

cDNA codon-optimized for E. coli; added N-termina…

Promoter

T7

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8

Plasmid

#224208

Purpose

Expresses human KDAC8 (HDAC8) in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Sci. 2015 Dec;24(12):2020-32. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Promoter

T5

Available Since

Dec. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLei-sfGFP-V2*D134*

Plasmid

#191111

Purpose

An E. coli sfGFP reporter with two TAG at positions 2 & 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Tags

His tag

Expression

Bacterial

Mutation

changing V2 & D134 to TAG

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392A)

Plasmid

#226980

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392F)

Plasmid

#226979

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392F)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392F

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(F401A)

Plasmid

#226978

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(F401A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

F401A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(L616E)

Plasmid

#226977

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(L616E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

L616E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226K)

Plasmid

#226976

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226K)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226K

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226M)

Plasmid

#226975

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226M)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226M

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226E)

Plasmid

#226974

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 7101 - 7120 of 9887 results

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