We narrowed to 7,720 results for: ALP

1. pLL3.7-EF-EYFP-YAP1_WT-PolyA

   Plasmid

   #112284

   Purpose

   Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   WT human YAP1-2gamma

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0656

   Plasmid

   #141653

   Purpose

   Lentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CHD3 (CHD3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2703

   Plasmid

   #144151

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ESRRA (ESRRA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP(N-glyc)-Tac-TC

   Plasmid

   #162496

   Purpose

   Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (TMD and cytosolic tail) (IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its TMD and cysotol…

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

   Plasmid

   #187957

   Purpose

   FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-KRAB-SpdCas9-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

   Plasmid

   #187953

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-KRAB-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1606

   Plasmid

   #143829

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BAZ2A (BAZ2A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Expression

   Bacterial

   Promoter

   ptetO

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-APPSw;T719N

   Plasmid

   #177094

   Purpose

   For lentiviral transduction of APPsw; T719N

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   APPsw;T719N (APP Human)
   

   Use

   Lentiviral

   Mutation

   T719N, K651N and M652L (please see depositor comm…

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL3.7-EF-EYFP-YAP1_S94A-PolyA

    Plasmid

    #112286

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with Serine 94 to Alanine

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Myc-HNF4A

    Plasmid

    #219396

    Purpose

    Express HNF4A in mammalian cells

    Depositor

    Yoichi Furukawa

    Article

    Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

    Insert

    HNF4A (HNF4A Human)
    

    Tags

    N terminal Myc tag

    Expression

    Mammalian

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATP1A1_plasmid_donor_RD

    Plasmid

    #86551

    Purpose

    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 (ATP1A1 Human)
    

    Use

    CRISPR and TALEN; Co-selection via hdr using ouab…

    Expression

    Mammalian

    Mutation

    Q118R, N129D

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6 and EF-1-alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-Hsp90 N(9-236)

    Plasmid

    #22481

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90 alpha N domain (HSP90AA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse av full length

    Plasmid

    #217809

    Purpose

    Full length mouse integrin av expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGAV (Itgav Mouse)
    

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV.EF1α.mEmerald.CD9.miR9T

    Plasmid

    #170452

    Purpose

    Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with microRNA 9 tandem cassette to restrict expression to microglia with EF1-alpha promoter.

    Depositor

    Tsuneya Ikezu

    Article

    Clayton et al Mol Neurodegener. 2021 Mar 22;16(1):18.

    Insert

    CD9 (CD9 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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