We narrowed to 2,435 results for: LRE

1. pAAV-FLEX-SaCas9-U6-sgGria3

   Plasmid

   #124855

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

   Plasmid

   #124865

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_4xhyb PylT A41AA C55A sfGFP 150TAG

   Plasmid

   #154772

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, hybrid PylT with A41AA an…

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

   Plasmid

   #91881

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Suz12 (Suz12 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pAG416GAL-ccdB-mRuby3

   Plasmid

   #166139

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNL-CEF-IgA1

   Plasmid

   #187009

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMTB2-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80066

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMTB2-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80067

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

    Plasmid

    #154777

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAA 150TAG in GFP reporter, hybrid PylT with …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PXPR007 sgATXN1L-1

    Plasmid

    #74962

    Purpose

    Cas9 + sgATXN1L-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PXPR007 sgATXN1L-2

    Plasmid

    #74963

    Purpose

    Cas9 + sgATXN1L-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ATXN1L (ATXN1L Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1 shETV1-A

    Plasmid

    #74973

    Purpose

    shETV1 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-EFS-hTSC1-T2A-Bsd

    Plasmid

    #193199

    Purpose

    Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sgRNA BRCA1 C64Y

    Plasmid

    #139326

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 C64Y using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1 shETV1-B

    Plasmid

    #74974

    Purpose

    shETV1 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_GP1Fc Cl13

    Plasmid

    #138599

    Purpose

    Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

    Depositor

    Annette Oxenius

    Article

    Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

    Insert

    LCMV GP1-Fc
    

    Use

    Tags

    Fc fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNL-CEF-IgM

    Plasmid

    #187013

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSicoR-sh4EBP2

    Plasmid

    #81121

    Purpose

    expresses an shRNA targeting murine 4E-BP2

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4E-BP2 shRNA (Eif4ebp2 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA

    Plasmid

    #51255

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Ala

    Promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST40-2XHA-Src-K298M-Y419F-Y530F

    Plasmid

    #140320

    Purpose

    Expresses HA-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    K298M-Y419F-Y530F

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

28. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

    Plasmid

    #87919

    Purpose

    HIV-1 SIN lentiviral transfer vector

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgHTT1, shGFP2, sgCas9, cherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1, U6, 7SK, PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

30. Lenti-sgNotch1#1/Cre

    Plasmid

    #193223

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch1#1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-sgNotch2#2/Cre

    Plasmid

    #193226

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#2 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

    Plasmid

    #67290

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Asc1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80059

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF5/FRT/DEST-3xHA/mGli2 P1-4A

    Plasmid

    #51252

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848 mutated to Ala

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. sgRNA BRCA1 Q1779Stop

    Plasmid

    #139331

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 Q1779Stop using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. sgRNA BRCA1 T922I

    Plasmid

    #139328

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 T922I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPRIME-CMV-GFP-shDyn2

    Plasmid

    #132712

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(2) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCR3-vsv-GCN4-dCEN-INCENP

    Plasmid

    #108510

    Purpose

    expression of vsv-GCN4-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    GCN4-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    replace CEN box for GCN4 dimerazation domain

    Promoter

    CMV

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDEST40-2XHA-Src-S32

    Plasmid

    #140308

    Purpose

    Expresses HA-tagged human Src-S32 in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Use

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AOI-WT-Cas9-sg-mouse Suz12-F2-GFP

    Plasmid

    #91882

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

47. DSPQ-HTT-TALEN2 BSD

    Plasmid

    #92246

    Purpose

    TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. DSPQ-HTT-TALEN1 ZEO

    Plasmid

    #92245

    Purpose

    TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pcDNA4/TO/GFPZNF598-9P-9A

    Plasmid

    #141192

    Purpose

    Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    ZNF598 (ZNF598 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    all 3 proline repeats are mutated to Alanine (9xP…

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

    Plasmid

    #104156

    Purpose

    luciferase reporter with ARHGAP29 mutant promoter

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 promoter (ARHGAP29 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    delete two TEAD binding sites

    Promoter

    ARHGAP29

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge