We narrowed to 15,697 results for: NOL
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pGS1222
Plasmid#63791PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection markerDepositorInserteGFP-Twin-Strep tag followed by URA3
UseE. coli cloning vectorTagseGFP-Twin-Strep tagPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
TagsNLSExpressionMammalianAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2623 (pCas6_Vch)
Plasmid#200895PurposeExpresses human codon-optimized V. cholerae Cas6 from a CMV promoter.DepositorInsertVchCas6
TagsNLSExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR6
Plasmid#118997PurposeTargeting vector for human AAVS1 with TLR6 reporter insertDepositorAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTn7·X-nia
Plasmid#122592PurposePlasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion siteDepositorInsertxylS/Pm->Nia
UseSynthetic Biology; Tn7 genomic integrationExpressionBacterialPromoterPmAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK7 IKK2/IKKb
Plasmid#26203DepositorAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Cor1-HA
Plasmid#102815PurposeExpression of Coronin1 in eukaryotic cellsDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGK112
Plasmid#228127PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ABE-CT
Plasmid#112876PurposeAAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9DepositorInsertABE-CT
UseAAVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS4A-2K-NS4B
Plasmid#120828PurposeEctopic expression of ZIKV proteinDepositorInsertNS4A-2K-NS4B ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL1B
Plasmid#67066PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-ACC1*
Plasmid#126740Purposeyeast plasmid overexpressing ACC1 from Komagataella phaffiiDepositorInsertacc1*
ExpressionYeastMutationT3452GPromoterGAPAvailable SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB450
Plasmid#74832PurposeGateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3045(gRNA XI-3)
Plasmid#73287PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA090
Plasmid#216099PurposeKnockout, EF1a-driven Cas12a (Cas only)DepositorInsertCas12a [EnAs]
UseCRISPR and Lentiviral; Assembled vectorAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-112
Plasmid#131127PurposeC-to-T base editing reporterDepositorInsertMutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
c3GIC9n
Plasmid#62192Purposecol1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettesDepositorInserthumanized S. Pyogenes Cas9 (D10A)
UseCRISPRTagsHAMutationD10A substitution (Cas9n)PromoterTRE3GAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only
