167,642 results

1. pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP

   Plasmid

   #213119

   Purpose

   Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   ActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. USP2

   Plasmid

   #36894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   USP2 (USP2 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pGP-AAV-syn-jGCaMP7s-WPRE (AAV Retrograde)

   Viral Prep

   #104487-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

   Plasmid

   #113865

   Purpose

   Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-iRFP
   PTCH1-mTurquoise2
   CBF-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-FLAG-BRAT1

   Plasmid

   #161920

   Purpose

   Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   BRCA1-associated ATM activator 1 (BRAT1 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-E-Syt3

   Plasmid

   #66832

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt3 (ESYT3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS puro HA-hUSP27X

   Plasmid

   #225718

   Purpose

   Bacterial expression of USP27X with HA tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X (USP27X Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSPneoHHgRNAH

   Plasmid

   #63557

   Purpose

   Expresses gRNA in Leishmania

   Depositor

   Greg Matlashewski

   Article

   Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

   Insert

   guide sequence
   

   Use

   Leishmania expression

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMET7-GAG-EGFP

   Plasmid

   #80605

   Purpose

   Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBL101gw

    Plasmid

    #199725

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    attR1/R2 cassette
    

    Expression

    Plant

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-coilin

    Plasmid

    #36906

    Depositor

    Greg Matera

    Article

    Shpargel et al J Cell Sci. 2003 Jan 15;116(Pt 2):303-12

    Insert

    coilin (COIL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL-CRISPR.EFS.tRFP

    Plasmid

    #57819

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSFFV-dNme2Cas9

    Plasmid

    #193190

    Purpose

    Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    dNme2Cas9
    

    Tags

    NLS and NLS-3xHA-NLS

    Expression

    Mammalian

    Mutation

    H588A, D16A

    Promoter

    SFFV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPIP_PepTile_40merFragments_65CancerDrivers

    Pooled Library

    #172109

    Purpose

    Lentiviral peptide expression library for overexpression fitness screening

    Depositor

    Prashant Mali

    Article

    Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

    Use

    Lentiviral

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pENTR5'-attP (pCM254)

    Plasmid

    #68311

    Purpose

    Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    attP
    

    Use

    Gateway entry vector

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Tags

    mKate

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJR89

    Plasmid

    #140096

    Purpose

    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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