We narrowed to 18,595 results for: MUT

1. p3xFLAG-CMV-Drosha (E419)

   Plasmid

   #171534

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Expression

   Mammalian

   Mutation

   a Drosha mutant stopping at E419

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-EGFP-DmTral-R42E_B

   Plasmid

   #145988

   Purpose

   Insect Expression of DmTral-R42E

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42E (tral Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations A198C, A387G and a mutation …

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP_∆R13–DD-talin1 (1-2299)

   Plasmid

   #166125

   Purpose

   For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   ∆R13–DD-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   residues 1-2299, deletion of dimerization domain …

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-Flag-Rheb-12Rs

   Plasmid

   #165023

   Purpose

   expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-12Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   All the lysine residues except K19 and K120 are c…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ST-Tstem-GFP

   Plasmid

   #162501

   Purpose

   Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The Tac stem region is inserted within ST6Gal1

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

   Plasmid

   #154778

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

   Plasmid

   #123340

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

   Plasmid

   #123344

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. B-HypaSpCas9

   Plasmid

   #126763

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A; M694A; Q695A; H698A; amino acids 1005-1013…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-FLAG-B-eSpCas9

    Plasmid

    #126772

    Purpose

    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AdMKP-3 S159AS197A

    Plasmid

    #131523

    Purpose

    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    changed Serine 159 to Alanine and Serine 197 to A…

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid

    #131111

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid

    #131112

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid

    #131113

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO-ORF66 C355A-2xStrep

    Plasmid

    #131114

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 355 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4/TO-ORF66 C344A-2xStrep

    Plasmid

    #131115

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 344 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO-ORF66 C341A-2xStrep

    Plasmid

    #131116

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 341 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO-ORF66 C301A-2xStrep

    Plasmid

    #131117

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 301 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO-ORF66 C298A-2xStrep

    Plasmid

    #131118

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 298 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO-ORF66 C295A-2xStrep

    Plasmid

    #131119

    Purpose

    Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

    Insert

    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    ORF66 a.a. 1-429 (full-length); cysteine 295 muta…

    Promoter

    CMV

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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