213,545 results

1. pJMP2846

   Plasmid

   #160676

   Purpose

   Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.3 Gag-imCherry

   Plasmid

   #208882

   Purpose

   codes for Gag from HIV-1 with an internal mCherry fluorescent protein

   Depositor

   Raphael Gaudin

   Article

   Diana Brychka et al bioRxiv

   Insert

   HIV-1 Gag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mNeonGreen-Lrrc59

   Plasmid

   #226564

   Purpose

   Encodes Lrrc59 protein labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL2-2xCLEAR-luciferase

   Plasmid

   #81120

   Purpose

   Reporter activity of the CLEAR network

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   2xCLEAR
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   2xCLEAR

   Available since

   Dec. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin-cpstFRET-Actin

   Plasmid

   #80643

   Purpose

   cpstFRET actin sensor for mechanical force measurements in cytoskeleton

   Depositor

   Fred Sachs

   Article

   Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

   Insert

   Beta actin, cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-GFP

   Plasmid

   #106281

   Purpose

   A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. NG-ABEmax

   Plasmid

   #124163

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   TadA-TadA(evo)-Cas9-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

   Plasmid

   #102935

   Purpose

   An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

   Depositor

   Brandon Harvey

   Article

   Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

   Insert

   NK1R (Tacr1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPCX-PECAMTS

   Plasmid

   #45850

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPCX-PECAMTS

    Plasmid

    #45850

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    PECAM1 (PECAM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid

    #212967

    Purpose

    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    

    Use

    CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, T7 and U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-dAPEX2

    Plasmid

    #117173

    Purpose

    Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    dAPEX2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2R

    Plasmid

    #167932

    Purpose

    lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS31: pTwinStrep-SUMO-PmcTnsAB

    Plasmid

    #168164

    Purpose

    PmcTnsAB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsAB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1973

    Plasmid

    #142519

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX3 (TBX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

    Plasmid

    #202762

    Purpose

    Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    NEUROG2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid

    #205618

    Purpose

    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    T28M, S455R- Please see depositor comment

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKrox24(MapErk)dTomato

    Plasmid

    #200113

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRSV-p65

    Plasmid

    #106453

    Purpose

    expression of NF-kB p65

    Depositor

    Georg Weber

    Article

    He et al Eur J Biochem. 2003 May . 270(10):2174-8

    Insert

    NF-kB p65 (RELA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE-PIK3CA-E545K

    Plasmid

    #116485

    Purpose

    Lentiviral expression of PIK3CA E545K

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E545K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NLS-QUE37C/pLBS CAG

    Plasmid

    #129322

    Purpose

    ATP measurement, nucleus, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBabe EGFR(T790M)

    Plasmid

    #32070

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T790M

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only