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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,552 results for: eng

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Showing: 761 - 780 of 14552 results

  5. v5 ABE-eVLP

    Plasmid
    #228467
    Purpose
    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs
    Depositor
    David Liu
    Publication
    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag(C507V)–ABE8e
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    MMLVgag(C507V)
    Promoter
    CMV
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    228467-plasmid-map-sequence-id-451030

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  13. acat 1-906

    Plasmid
    #24194
    Depositor
    David Rimm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha catenin (CTNNA1 Human)
    Expression
    Mammalian
    Mutation
    Full length
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    24194-plasmid-map-sequence-id-240248

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  16. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65772-plasmid-map-sequence-id-112654

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  18. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Publication
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133451-plasmid-map-sequence-id-267048

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  19. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid
    #71293
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Publication
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71293-plasmid-map-sequence-id-127936

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  20. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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Showing: 761 - 780 of 14552 results