We narrowed to 47,577 results for: nes

1. pAAV-CaMKII-iGECI-NES

   Plasmid

   #160424

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Akt-STOPS-NES

   Plasmid

   #175008

   Purpose

   Cytosol-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACt-mCherry-NES

   Plasmid

   #138215

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NES (Adcy10 Rat)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 863 pSG5L HA NES PTEN

   Plasmid

   #10932

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Tags

   HA and NES

   Expression

   Mammalian

   Available Since

   Jan. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGG-NES-TurboID-GFP

   Plasmid

   #209401

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   NES-3XHA-TurboID
   

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-NES-YFP-CCTc

   Plasmid

   #184328

   Purpose

   Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)

   Depositor

   Xiaodong Liu

   Article

   Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

   Insert

   CCTc (CACNA1C Human)
   

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSIIpuro-4493NES(Booster-PKA)

   Plasmid

   #184710

   Purpose

   PKA biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   Booster-PKA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBbsr-6080NES(Epac-sensor)

    Plasmid

    #162487

    Purpose

    hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Epac-sensor (RAPGEF3 Synthetic, Human)
    

    Tags

    YPet and Turquoise2GL

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH2B-SCFP1-2A-mTq2-NES

    Plasmid

    #60491

    Purpose

    Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

    Depositor

    Dorus Gadella

    Article

    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

    Inserts

    H2B-SCFP1
    mTurquoise2-NES
    

    Tags

    SCFP1 and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-NPM NESM-NLS1D

    Plasmid

    #13288

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L100A, L102A, deletion of aa134-aa142

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-NES-fRGECO1

    Plasmid

    #125247

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Tags

    NES signal

    Expression

    Mammalian

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

    Plasmid

    #194688

    Purpose

    hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_04-mEGFP
    

    Use

    AAV

    Tags

    mEGFP

    Promoter

    hSyn1

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Coexpressed Free GFP-NES

    Plasmid

    #182491

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    GFP-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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