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164,102 results

Showing: 801 - 820 of 164102 results

  1. FLT3-ITD-MSCV-IRES-GFP

    Plasmid
    #184779
    Purpose
    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells
    Depositor
    Timothy Ley
    Insert
    FLT3 (FLT3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Activating internal tandem duplication (ITD) adde…
    Promoter
    MSCV
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184779-plasmid-map-sequence-id-364900

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  2. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

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  3. pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)

    Viral Prep
    #105669-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    CaMKII
    Tags
    FusionRed
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  4. pENN.AAV.CMVs.Pl.Cre.rBG (AAV9)

    Viral Prep
    #105537-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    None
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  5. pDV002

    Plasmid
    #191823
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191823-plasmid-map-sequence-id-375876

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  10. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

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  11. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV9)

    Viral Prep
    #55645-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  13. pCW-Cas9-Blast

    Plasmid
    #83481
    Purpose
    Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA
    Depositor
    Mohan Babu
    Inserts
    SpCas9
    Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Replaced puromycin N-acetyltransferase on the ori…
    Promoter
    Tight Tre and human phosphoglycerate kinase 1 pro…
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83481-plasmid-map-sequence-id-335118

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  14. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-GFPnanobody
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214837-plasmid-map-sequence-id-425310

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  17. pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)

    Viral Prep
    #44362-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv 2024.07.11.602973
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Use
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

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Showing: 801 - 820 of 164102 results