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Plasmid#221198PurposeScChd1-aa118-1274 chromatin remodeler with Y137A mutationDepositorInsertScChd1 (aa118-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationY137APromoterT7Available sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[∆ChEx]
Plasmid#221199PurposeScChd1-aa142-1274 chromatin remodeler with ChEx domain deletedDepositorInsertScChd1 (aa142-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-3xFLAG-Ubc7
Plasmid#212801PurposeConstitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cellsDepositorInsertUbc7
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0520 (pDEST, TRP1)
Plasmid#210481PurposepDEST vector with TRP1 marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseDestination vector for gateway cloningTagsExpressionMutationPromoterADH1Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:TDimer mVenus
Plasmid#210520PurposeExpresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertTandem dimer mVenus
UseZebrafish expression, tol2 kit gateway-compatibleTagsExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:R198P mVenus
Plasmid#210519PurposeExpresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationR198PPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest-Cre
Plasmid#202714PurposeCre recombinase staging plasmid to make transposon insertion libraries.DepositorInsertCre
UseStaging plasmid to make transposon insertion libr…TagsExpressionMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-HO1(51-231)
Plasmid#203486PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.DepositorInsertArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-AERRIE
Plasmid#204023PurposeLentiviral vector expressing human long non-coding RNA AERRIE (linc01013)DepositorInsertlong non-coding RNA AERRIE (LINC01013 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/TO/V5-DEST-mTurquoise2
Plasmid#191010PurposeLentiviral expression vector for mTurquoise2DepositorInsertmTurquoise2
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Ctdnep1_C-ter
Plasmid#196525PurposeExpression of Ctdnep1_C-terDepositorInsertCtdnep1-C-ter (CTDNEP1 Human)
UseTagsHisExpressionBacterialMutationNo transmembrane domainPromoterT7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDEST40-CES2C-ΔC-S230A
Plasmid#200557PurposeMammalian expression of secreted mouse CES2C S230A mutantDepositorInsertMouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_1-183
Plasmid#196535PurposeExpression of GST-Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_181-299
Plasmid#196536PurposeExpression of GST-Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_544-715
Plasmid#196540PurposeExpression of GST-Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_297-370
Plasmid#196537PurposeExpression of GST-Eps8L2_297-370DepositorInsertEps8L2_297-370 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_367-496
Plasmid#196538PurposeExpression of GST-Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_494-546
Plasmid#196539PurposeExpression of GST-Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoter; lac UV5 promoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-LASSIE
Plasmid#192105PurposeLentiviral vector expressing long non-coding RNA LASSIE (linc00520)DepositorInsertLASSIE
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.2/V5-DEST-Noa1
Plasmid#191969PurposeOverexpression of Nitric Oxide Associated 1 protein (NOA1), tagged with V5.DepositorInsertNoa1
UseSynthetic BiologyTagsV5ExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA-HA
Plasmid#186374PurposeDestination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-HA-RfA
Plasmid#186373PurposeDestination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe3-RfA
Plasmid#186370PurposeDestination vector for the expression of a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST_FLAG-HUWE1_delta169-189
Plasmid#187136Purposemammalian cell expression of FLAG-HUWE1 delta169-189DepositorInsertHUWE1 (HUWE1 Human)
UseTags1xFLAGExpressionMammalianMutationdelta169-189PromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain
Plasmid#183387PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertredesigned Caspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationANR261ENP, D315H, M318K, T352KPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_grh-1p-grh-1-gfp
Plasmid#177761PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-dre4
Plasmid#170440PurposeExpress drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.DepositorInsertdre4 (dre4 Fly)
UseTags6xHis and V5ExpressionBacterialMutationDeleted amino acids 1-20PromoterT7Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorInsertssrp1 (Ssrp Fly)
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX-0SB
Plasmid#169730PurposeBacterial expression of N-terminally 6His tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTags6 HisExpressionBacterialMutationComprises residues 495-741 and has the major SB m…PromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 527-PHL2
Plasmid#118670PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 527-XERICO
Plasmid#118671PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 544-PHL2
Plasmid#118672PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6-NusAExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 544-XERICO
Plasmid#118673PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6-NusAExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 565-PHL2
Plasmid#118674PurposeBacterial overexpressionDepositorInsertPHL2 (AT3G24120 Mustard Weed)
UseTagsHis6-GSTExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST 565-XERICO
Plasmid#118675PurposeBacterial overexpressionDepositorInsertXERICO (XERICO Mustard Weed)
UseTagsHis6-GSTExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only