We narrowed to 14,414 results for: cas9

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ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY)

Plasmid

#251545

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_L34W-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx2 L34W

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT)

Plasmid

#251551

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY)

Plasmid

#251547

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_WT_GFP (ABE8e_V28C_nCas9_WT)

Plasmid

#251548

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT)

Plasmid

#251550

Purpose

Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_WT

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY)

Plasmid

#251546

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_M151E-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx3 M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W)

Plasmid

#251552

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY_V106W

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY)

Plasmid

#251544

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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dCas9-KYP (M6)

Plasmid

#239480

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA methylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-dCas9-2XNLS-XTEN16-BFP-KYP

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-ryt

Plasmid

#246022

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-tra135

Plasmid

#246023

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-tra246

Plasmid

#246024

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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EML8357_pA3A-CBECas9

Plasmid

#234352

Purpose

A base editor derived by egg-cell specific promoter

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9

Plasmid

#248566

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV, TetO2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS

Plasmid

#244835

Purpose

Bacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpRY Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 71 cerulean

Plasmid

#230954

Purpose

aTc inducible knockdown of FliZ and CRP

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pKDcas9 13 cerulean

Plasmid

#230955

Purpose

aTc inducible knockdown of CRP and HNS

Depositor

Andrew Griffiths

Article

Deyell et al iScience. 2024 Dec 9;28(1):111521. doi:

Insert

dCas9

Use

CRISPR

Mutation

additional tetO

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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