We narrowed to 13,810 results for: cas9 genes

1. pSEVA47_dCas9-GFP_NOT_iC

   Plasmid

   #231418

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-tdTomato

   Plasmid

   #232631

   Purpose

   catalytically inactive dCas9 for localization; labeled with tdTomato

   Depositor

   Karsten Rippe

   Insert

   dCas9-tdTomato
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA47_dCas9-GFP_NOT_i1

   Plasmid

   #231416

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

   Plasmid

   #222988

   Purpose

   Bacterial expression of iGeoCas9(C2) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9-hGeminin

   Plasmid

   #199344

   Purpose

   modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin

   Depositor

   Iain Hagan

   Article

   Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1

   Insert

   ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
   

   Use

   Tags

   cas9 c-term fused to hgemenin 1-110 fragment

   Expression

   Mammalian

   Mutation

   cas9 c-term fused to hgemenin 1-110 fragment

   Promoter

   cbh

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-miniCGBE1

   Plasmid

   #198548

   Purpose

   Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-NG-miniCGBE1

   Plasmid

   #198538

   Purpose

   Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NRRH

    Plasmid

    #198540

    Purpose

    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRRH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH

    Plasmid

    #198542

    Purpose

    Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-FCPF

    Plasmid

    #220131

    Purpose

    Expresses dCas9-FCPF in mammalian cells

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA1_Cas9-hGem_Smc4

    Plasmid

    #217667

    Purpose

    expresses Cas9-hGem and guideRNA for tagging of SMC4

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Cas9-NS-A

    Plasmid

    #207823

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-Cas9-NS-B

    Plasmid

    #207824

    Purpose

    Dual expression of Cas9 and non-specific sgRNA

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    non-specific sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnCas9-AID

    Plasmid

    #196242

    Purpose

    Plasmid expressing the nCas9-AID fusion

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    nickase Cas9 fused to AID
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-evoSpCas9-E526K

    Plasmid

    #207365

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9-E526K
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    M495V, Y515N, R661Q, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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