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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,783 results for: cloning

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Showing: 801 - 820 of 63783 results

  3. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

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  4. pMJ920

    Plasmid
    #42234
    Depositor
    Jennifer Doudna
    Publication
    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Tags
    GFP, HA epitope, and NLS (nuclear localization si…
    Expression
    Mammalian
    Mutation
    codon-optimized synthetic DNA sequence
    Promoter
    CMV
    Available since
    March 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42234-plasmid-map-sequence-id-57040

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  7. pLEX_305

    Plasmid
    #41390
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Gateway Cloning and Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41390-plasmid-map-sequence-id-352470

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  14. pdCas9-Tet1-CD

    Plasmid
    #83340
    Purpose
    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system
    Depositor
    Ronggui Hu
    Publication
    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    U6, CBH
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83340-plasmid-map-sequence-id-334608

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  15. pLV-TetO-hNGN2-Puro

    Plasmid
    #79049
    Purpose
    Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15
    Insert
    hNGN2-P2A-PuroR (NEUROG2 Human)
    Use
    Lentiviral
    Promoter
    TetO
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79049-plasmid-map-sequence-id-429069

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Showing: 801 - 820 of 63783 results