167,768 results

1. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB533-44B12m23-sfGFP

   Plasmid

   #186778

   Purpose

   RNAP2 Ser5ph-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   44B12m23-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC-NRP1

   Plasmid

   #158384

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   NRP1 (NRP1 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAF0474 Ef1a-Si74_attB acceptor

   Plasmid

   #193475

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74_attB
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD3_mCherry

   Plasmid

   #187385

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-NL1(-)

   Plasmid

   #15260

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 (Nlgn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-mEGFP

   Plasmid

   #182876

   Purpose

   Localization: Endoplasmic Reticulum

   Depositor

   Salvatore Chiantia

   Article

   Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

   Insert

   ER-mEGFP
   

   Expression

   Mammalian

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CamKIIa-jGCaMP8m-WPRE (AAV5)

   Viral Prep

   #176751-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CamKIIalpha

   Available Since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

9. Fuw-dCas9-Tet1CD-P2A-BFP

   Plasmid

   #108245

   Purpose

   Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A

   Promoter

   Ubc

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pFRT/TO/HIS/FLAG/HA-PGK1

    Plasmid

    #38071

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. myc-KIF5B

    Plasmid

    #127617

    Purpose

    N-terminal myc-tagged mouse KIF5B

    Depositor

    Josef Kittler

    Article

    Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

    Insert

    KIF5B (Kif5b Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304-IRES-GFP

    Plasmid

    #176288

    Purpose

    pLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sites

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

    Plasmid

    #214102

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    PE-Plus
    

    Expression

    Mammalian

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-NLS-HA-Dre

    Plasmid

    #51272

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Dre
    

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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