We narrowed to 16,248 results for: ENA

Showing: 8501 - 8520 of 16248 results

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pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

Plasmid

#133560

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcU6_3 sgRNA

Plasmid

#92359

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pDawn-Ag43

Plasmid

#107742

Purpose

Expresses Ag43 under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCF827-recipient_U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Plasmid

#211692

Purpose

U6-sgRNA-EFS-Cas9-P2A-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pIII pm

Plasmid

#218100

Purpose

Phagemid that expresses LacI, and pIII under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pIII

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-parkin W403A

Plasmid

#45876

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

W403A mutation

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
hBACCS2-IRES-mCherry

Plasmid

#72892

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a-EGFP

Plasmid

#205309

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-phiC31-LP-TC

Plasmid

#183755

Purpose

AAVS1 targeting construct with the phiC31 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

FRT-mCherry-attP-BleoR-F3

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_EGFP

Plasmid

#73836

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-p58

Plasmid

#166942

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

p58 (Lman1 Rat)

Tags

GFP and chicken lysozyme signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (full-length, aa 1-293)

Plasmid

#61418

Purpose

Expresses human OTUD6B (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

Plasmid

#196678

Purpose

AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

PKIαmut-P2A-mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

Plasmid

#193752

Purpose

human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-Turbo-YFP-NLS-STOP-L2

Plasmid

#127361

Purpose

gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPseq-BFP-backbone

Plasmid

#85707

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pafG-CBE-3T

Plasmid

#220374

Purpose

for hA3A-nCas9 and gRNA expression in Aspergillus flavus

Depositor

Xue Gao

Article

Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

Inserts

hA3A-nCas9
tRNA and gRNA scaffold

Use

CRISPR

Promoter

Ptef1 and tRNA

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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