We narrowed to 2,403 results for: T2A

1. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HIV7-Bcl2-CD28-CD3z-T2A-EGFR

   Plasmid

   #163465

   Purpose

   Lentiviral vector encoding a Bim-specific chimeric antigen receptor with a synthetic Bcl2 binding element

   Depositor

   David Baker

   Article

   Lajoie et al Science. 2020 Sep 25;369(6511):1637-1643

   Insert

   Bim-specific CAR
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-AU1

   Plasmid

   #162111

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG

   Plasmid

   #162090

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

   Plasmid

   #162097

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-AU1

   Plasmid

   #162082

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-StrepTagII-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-StrepTagII-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SOX9-T2A-H2B-EGFP repair template

   Plasmid

   #167972

   Purpose

   SOX9 reporter repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   SRY-Box Transcription Factor 9 (SOX9 Human)
   

   Use

   CRISPR; Donor template

   Tags

   H2B-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p194-RV-CAG-dio-mScarlet-T2A-Brn2

    Plasmid

    #204741

    Purpose

    Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    Brn2
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rogi2-CMV-LAMB3-T2A-GFP-bGH

    Plasmid

    #179865

    Purpose

    Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

    Depositor

    George Church

    Article

    Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

    Insert

    LAMB3-T2A-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-AU1

    Plasmid

    #162102

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-StrepTagII-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

    Plasmid

    #162104

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-FLAG

    Plasmid

    #162110

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

    Plasmid

    #162085

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-ProtC-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-ProtC-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

    Plasmid

    #162088

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-FLAG-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-FLAG-AU1

    Plasmid

    #162094

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-FLAG-AU1
    

    Use

    Lentiviral

    Tags

    StrepTagII-FLAG-AU1

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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