Addgene

We narrowed to 8,751 results for: ef1

Showing: 841 - 860 of 8751 results

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI EF1alpha FLAG/HA-PALI1

Plasmid

#114445

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmCherry-mAID

Plasmid

#140603

Purpose

Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo

Plasmid

#128453

Purpose

lentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-jGCaMP8s-WPRE

Plasmid

#176758

Purpose

AAV-mediated expression of GCaMP8s under the EF1a promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344A-mCerulean

Plasmid

#69580

Purpose

Expresses p53-L344A mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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p156RRL-EF1a-GFP-U3H1SatA

Plasmid

#41795

Depositor

Inder Verma

Article

Zhu et al Nature. 2011 Sep 7;477(7363):179-84. doi

Insert

satellite repeat sequence

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X[EF1α-copGFP]

Plasmid

#218323

Purpose

Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

copGFP mammalian expression cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-DHB-mTurq2

Plasmid

#212667

Purpose

Express tagged DHB

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

DHB (HELB Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mutation

Promoter

EF-1a

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA Human, human)

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-RandE3

Plasmid

#79872

Purpose

For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Fon-tdTomato

Plasmid

#72261

Purpose

Express tdTomato when FLP is expressed by another vector

Depositor

Kazuhiro Oka

Insert

tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T230I in tdTomato (please see depositor's co…

Promoter

elongation factor-1

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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TRE-STOP EF1a-rtTA3

Plasmid

#195882

Purpose

control construct of TRE-VEGFA EF1a-rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Synthetic, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-GRAB_NPY1.0

Plasmid

#208677

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Use

AAV

Tags

Expression

Mutation

Promoter

EF1_

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5(TA)-ires-puro

Plasmid

#167823

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5(TA)

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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