We narrowed to 17,485 results for: puro

1. pLenti-gRNA-puro

   Plasmid

   #180426

   Purpose

   Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

   Insert

   gRNAscr
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Tight-Puro_Nkx6.2

   Plasmid

   #64846

   Purpose

   A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx6.2 (Nkx6-2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-CCND1-HA-puro

   Plasmid

   #212670

   Purpose

   Express tagged cyclin D1

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-puro-a5 guide B

   Plasmid

   #194011

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-puro-aV guide A

   Plasmid

   #194008

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9n(BB)-2A-puro-aV guide B

   Plasmid

   #194009

   Purpose

   For CRISPR knockout of integrin alphaV

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (aV_guide B)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9n(BB)-2A-puro-a5 guide A

   Plasmid

   #194010

   Purpose

   For CRISPR knockout of integrin alpha5

   Depositor

   Timothy Springer

   Article

   Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

   Insert

   single-guide RNA (a5_guide A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSITEP-puro-shWRN1

   Plasmid

   #125783

   Purpose

   DOX-inducible expression of a short-hairpin RNA targeting human WRN

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   shWRN1 (WRN Human)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Puro-moxGFP

    Plasmid

    #207688

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBabepuro3-p18 A61D

    Plasmid

    #24937

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    p18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A61D

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pLKO.1-puro-shRFP

    Plasmid

    #125778

    Purpose

    constitutive expression of a short-hairpin RNA targeting RFP

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX307-CTSL-puro

    Plasmid

    #158466

    Purpose

    Constitutive lentiviral expression of human CTSL.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSL (CTSL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3puro-EGFP-GAPDH

    Plasmid

    #225475

    Purpose

    Express mEGFP-fusion protein of GAPDH

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    GAPDH (GAPDH Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-P2A-CIAR_pLenti_CMV_Puro

    Plasmid

    #86503

    Purpose

    Lenti plasmid for generating CIAR expressing stable cell lines

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    GFP-P2A-CIAR (SOS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T968L in SOScat

    Promoter

    CMV

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABEpuro-ERBB2 S310Y

    Plasmid

    #40992

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S310Y

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. DNA-CARtcr-IRESpuro

    Plasmid

    #89346

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5LTR-puro-3LTR

    Plasmid

    #86948

    Purpose

    PiggyBac transposon

    Depositor

    Rob Mitra

    Article

    Qi et al Nucleic Acids Res. 2017 Jan 12. pii: gkw

    Insert

    PiggyBac transposon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. BAF-WT-tet-puro

    Plasmid

    #199648

    Purpose

    Doxycycline-inducible BAF expression

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    BAF (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-puro-sh-mFAK B

    Plasmid

    #37018

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    sh-mFAK B (Ptk2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only