167,825 results

1. pET12a-PAPI

   Plasmid

   #19961

   Depositor

   Jonathan Tait

   Article

   Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

   Insert

   Annexin V (ANXA5 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Actinin-sstFRET-GR

   Plasmid

   #83416

   Purpose

   FRET-based actinin tension sensor

   Depositor

   Tetsuya Kitaguchi

   Article

   Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

   Insert

   Actinin-sstFRET-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

3. FLAG.PKCepsilon.K/W

   Plasmid

   #10796

   Depositor

   Alex Toker

   Article

   Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

   Insert

   PKC epsilon (PRKCE Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K437W (Lys 437 to Trp)

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

4. scAAV-sgRNA-GFP

   Plasmid

   #177935

   Purpose

   Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET20b- hTPI

   Plasmid

   #50723

   Purpose

   overexpression of human TPI1 in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Tags

   6xHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHMattR3-attR4-GFP

   Plasmid

   #196247

   Purpose

   pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Calnexin-HT8-mGold

   Plasmid

   #183989

   Purpose

   Expresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   Calnexin (CANX Human)
   

   Tags

   HaloTag8 (C61S, C262S), mGold

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

   Plasmid

   #216156

   Purpose

   Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with C-terminal FLAG tag
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-Fascin-C-10

   Plasmid

   #54094

   Purpose

   Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep

    #100851-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 (#100851). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

13. ER-mEGFP

    Plasmid

    #182876

    Purpose

    Localization: Endoplasmic Reticulum

    Depositor

    Salvatore Chiantia

    Article

    Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

    Insert

    ER-mEGFP
    

    Expression

    Mammalian

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Expression

    Bacterial

    Promoter

    glnS' and lambda(ts) and proK

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Mutation

    truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1NB m Rosella I

    Plasmid

    #71247

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Tags

    yeast mitochondrial targeting sequence of citrate…

    Expression

    Yeast

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1_Zeo_TetO_FLAG-AT1R

    Plasmid

    #222856

    Purpose

    Mammalian expression plasmid for FLAG-tagged AT1R

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Spectrin-M-cpstFRET

    Plasmid

    #61109

    Purpose

    Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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