161,697 results

1. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. iGFP-beta-actin

   Plasmid

   #231553

   Purpose

   Mammalian expression of human beta actin fused intramolecularly to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP (intramolecular)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-GFP

   Plasmid

   #181906

   Purpose

   Fluorescently tagged dead Cas9

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMasterE

   Plasmid

   #165080

   Purpose

   Episomal expression of reprogramming factors.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   MYC (MYC Human)
   LRH1 (NR5A2 Human)
   NANOG (NANOG Human)
   LIN28 (LIN28A Human)
   EGFP
   HSV TK
   

   Use

   Episomal

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NET47 pEGFP-N2 (593)

   Plasmid

   #61986

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET47 (TM7SF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pLenti 4.1 Ex miR200c-141

   Plasmid

   #35534

   Purpose

   3rd generation lentiviral vector

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   miR200c-141 genomic cluster (MIR200C, MIR141 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2181

   Plasmid

   #158421

   Purpose

   AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple32 (CLDN5 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple32

   Available since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tet-O-FUW-Brn2

    Plasmid

    #27151

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-14-3-3 eta

    Plasmid

    #116887

    Purpose

    Expresses HA-tagged human 14-3-3 eta in mammalian cells

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Article

    Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

    Insert

    14-3-3 eta (YWHAH Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2-nbt-mTagBFP2-P2A-mScarlet

    Plasmid

    #196115

    Purpose

    zebrafish expression of nbt-mTagBFP2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-mTagBFP2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Tol2

    Plasmid

    #31823

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-CMV-Galpha-i1 ONE-GO

    Plasmid

    #189735

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i1 ONE-GO
    GNAI1 (Gnai1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. eIF2a 1

    Plasmid

    #21807

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML433

    Plasmid

    #177190

    Purpose

    Expresses recombinant HyPro enzyme in E. coli

    Depositor

    Eugene Makeyev

    Article

    Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

    Insert

    HyPro enzyme
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ORAI1-hBACCS2

    Plasmid

    #67626

    Purpose

    Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    ORAI1-hBACCS2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPR101-Tango

    Plasmid

    #66301

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR101 (GPR101 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pHCMV_GP64

    Plasmid

    #207276

    Purpose

    Mammalian expression of baculovirus GP64 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    GP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

    Plasmid

    #84468

    Purpose

    pAAV-CRAM-tdTomato

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    T230I (please see depositor comments below)

    Promoter

    TRE

    Available since

    Dec. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge