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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,102 results for: Eras

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Showing: 8681 - 8700 of 42102 results

  2. dCas9-NLS-gs10-cDHFR174

    Plasmid
    #188256
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split DHFR (aa 1-173), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cDHFR174 (DHFR Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188256-plasmid-map-sequence-id-374594

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  4. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  9. K15-TK/pGL3

    Plasmid
    #44267
    Depositor
    George Cotsarelis
    Article
    Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Mouse Targeting; Thymidine kinase
    Tags
    HSV-1 TK
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. psicheck-2-LPPRC-mod

    Plasmid
    #48168
    Purpose
    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element
    Depositor
    Vamsi Mootha
    Article
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Insert
    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    Use
    Luciferase
    Mutation
    A/T mutation in the ATG of the uORF
    Available Since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid
    #155059
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155059-plasmid-map-sequence-id-308442

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Showing: 8681 - 8700 of 42102 results