178,426 results
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Plasmid#83375PurposeExpression of Myc tagged GFPDepositorInsertMyc-GFP
TagsMycExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYES2-HTH
Plasmid#86470PurposeYeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoterDepositorTypeEmpty backboneTagsHIS10-TEV-HAExpressionYeastPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_TOMM20_sfCherry2(11)
Plasmid#83033PurposeExpresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cellsDepositorInsertTOMM20_sfCherry+11
ExpressionMammalianAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCytERM_mScarlet-i_N1
Plasmid#85068PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
TagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGH153_MS2-AIDΔ-Hygro
Plasmid#85416Purposelentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable markerDepositorInsertMS2-AIDΔ and Hygromycin resistance
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-P-CAG-GFPluc2
Plasmid#80493Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expressionDepositorInsertGFPluc2
UseDonor vector (human)ExpressionMammalianPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV Retrograde, AAV1, AAV2, AAV8, and AAV9InserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLIB
Plasmid#80610PurposepLIB - Library vector (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectPromoterpolyhedrinAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL_U2AF1_WT_mCherry
Plasmid#84017Purposeexpression of U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMART-sgRNA (Sp)
Plasmid#80427PurposeU6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.DepositorTypeEmpty backboneExpressionMammalianAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PR_GEF YFP (2XPDZ-YFP-LARG DH)
Plasmid#80408PurposeRecruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-Gsdmd-CT
Plasmid#80953PurposeExpresses Gsdmd-CT in mammalian cellsDepositorAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hHSF1 WT
Plasmid#71724Purposeplasmid expressing myc-his tag hHSF1 WTDepositorAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3-STAT3-S727D
Plasmid#73364PurposeExpression of STAT3-S727DDepositorInsertSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
TagsFLAG taggedExpressionMammalianMutationE16K S727DPromoterCMVAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcFLAG-FYN-WT
Plasmid#74509PurposeOverexpression of FYN in mammalian cellsDepositorAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-Flag-CDK19-WT-IRES-ZsGreen
Plasmid#68858PurposeExpresses Human CDK19 Wild-TypeDepositorInsertCyclin-Dependent Kinase 19 (CDK19 Human)
UseLentiviralTagsFlagExpressionMammalianPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Glycine max, Mouse)
Tagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
C321.deltaA (Isaacs lab)
Bacterial strain#73581PurposeGenomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB geneDepositorBacterial resistanceAmpicillinAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-flex-dsRed-shvgat
Plasmid#67845PurposeExpresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switchDepositorInsertAAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiExpressionMammalianPromoterh-synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by