164,060 results

1. pWZL-hygro-FAKWT

   Plasmid

   #216540

   Purpose

   This retroviral plasmid expresses the human wild type PTK2

   Depositor

   Georgia Konstantinidou

   Article

   Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

   Insert

   PTK2 (PTK2 Human)
   

   Use

   Retroviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   wild type sequence

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUA66 theta GFP

   Plasmid

   #105607

   Purpose

   Expresses GFP with no promoter

   Depositor

   Pamela Silver

   Article

   Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   No Promoter

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExpreS2-1-C3-10H-SIII

   Plasmid

   #175444

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-10His-TwinStrep and BiP Secretion Peptide

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.R_TRE_minCMV

   Plasmid

   #211518

   Purpose

   Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

   Depositor

   Justin English

   Article

   Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SV40 and minCMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-dCas9-EGFP-HaloTag

   Plasmid

   #169451

   Purpose

   expression of dCas9-EGFP-HaloTag

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   dCas9-EGFP-HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 CMV 3xFG eIF4G1

   Plasmid

   #158775

   Purpose

   pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-SOCS1

   Plasmid

   #174571

   Purpose

   Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   HA-SOCS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP_CD47_LU

    Plasmid

    #65474

    Purpose

    GFP tagged CD47 with its own long 3'UTR

    Depositor

    Christine Mayr

    Article

    Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

    Inserts

    Signal peptide of CD47
    GFP
    CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Use

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

    Plasmid

    #35503

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2(C128S/D156A)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    Ef1a

    Available since

    April 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. IL-2 promoter luciferase

    Plasmid

    #12194

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-2 promoter (Il2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-C16orf80-flag

    Plasmid

    #70645

    Purpose

    Expresses C16orf80 in mammalian cells with FLAG-tag epitope.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    C16orf80 (CFAP20 Human)
    

    Use

    Retroviral

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pT3-EF1a-Bmi1

    Plasmid

    #31783

    Depositor

    Xin Chen

    Article

    Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. B2345_BTB2x-mCherry

    Plasmid

    #215097

    Purpose

    Expresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BCL6 BTB-BTB (BCL6 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-DO-ChroME2f-ST-P2A-GCaMP8s

    Plasmid

    #227786

    Purpose

    Cre off - Cation channelrhodopsin ChroME2f fused to GCaMP8m and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Insert

    DO-ChroME2f-ST-P2A-GCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-IRF4

    Plasmid

    #58987

    Purpose

    MSCV based expression vector - co-expresses GFP and murine IRF4

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    Mouse IRF4 (Irf4 Mouse)
    

    Use

    Retroviral

    Tags

    Kozak sequence added

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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