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Showing: 881 - 900 of 26710 results

  2. DF-UAS-spa-GCaMP6f

    Plasmid
    #67568
    Purpose
    Zebrafish expression of a photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    HIS-tag
    Expression
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV/CaMKIIa::iLMO2

    Plasmid
    #129481
    Purpose
    AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter
    Depositor
    Robert Gross
    Article
    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10
    Insert
    iLMO2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    129481-plasmid-map-sequence-id-385172

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  7. pAAV-cFos-XRI-V5

    Plasmid
    #178058
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-V5
    Use
    AAV
    Tags
    V5-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Mouse c-fos promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178058-plasmid-map-sequence-id-353602

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  11. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  15. pAAV-UBC-XRI-HA

    Plasmid
    #178056
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-HA
    Use
    AAV
    Tags
    HA-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178056-plasmid-map-sequence-id-349205

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  17. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Kensaku Sakamoto
    Article
    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197101-plasmid-map-sequence-id-390718

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  19. pQE80L-SpyCatcher-ELP-GFP

    Plasmid
    #69835
    Purpose
    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do
    Insert
    SpyCatcher-ELP-GFP
    Use
    Tags
    6x His Tag, GFP, and TEV Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5 promoter/lac operator element
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    69835-plasmid-map-sequence-id-183106

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  20. pssGFP-hCRT

    Plasmid
    #80022
    Purpose
    Mammalian expression of signal peptide-EGFP-humanCRT
    Depositor
    Chinten James Lim
    Article
    Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi
    Insert
    CRT (CALR Human)
    Use
    Tags
    EGFP and signal peptide
    Expression
    Mammalian
    Mutation
    signal peptide 17aa added before GFP-CRT, will ta…
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 881 - 900 of 26710 results