167,785 results

1. EasyFusion T2A-H2B-mCherry

   Plasmid

   #113089

   Purpose

   General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-mCherry
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTetON-EGFP

   Plasmid

   #89453

   Purpose

   Tet/Dox-inducible expression of EGFP in mammalian cells (control)

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-GB

   Plasmid

   #60520

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-APOE-E4-T2A-mCherry

   Plasmid

   #190809

   Purpose

   Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Insert

   APOE (APOE Human)
   

   Use

   Lentiviral

   Mutation

   E4

   Promoter

   Ubi C

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSyn.flex.H2B.GFP

   Plasmid

   #170368

   Purpose

   Cre-inducible AAV vector expressing nuclear GFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   flex H2B GFP
   

   Use

   AAV

   Promoter

   human Synapsin

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-ZDHHC11

   Plasmid

   #231659

   Purpose

   Expresses human ZDHHC11 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC11 (ZDHHC11 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. EasyFusion T2A-H2B-GFP

   Plasmid

   #112851

   Purpose

   General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   T2A-H2B-GFP
   

   Use

   Synthetic Biology

   Promoter

   No

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   synapsin I

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD4_mCherry

    Plasmid

    #187386

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-ZDHHC17

    Plasmid

    #231665

    Purpose

    Expresses human ZDHHC17protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC17 (ZDHHC17 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-EGFP

    Plasmid

    #86639

    Purpose

    Expresses mCherry-eGFP tandem (cytoplasmic)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    mCherry-EGFP
    

    Tags

    mCherry-EGFP

    Expression

    Mammalian

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-ZDHHC13

    Plasmid

    #231661

    Purpose

    Expresses human ZDHHC13 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC13 (ZDHHC13 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-ZDHHC18

    Plasmid

    #231666

    Purpose

    Expresses human ZDHHC18 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC18 (ZDHHC18 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG36E

    Plasmid

    #133853

    Purpose

    Expression vector

    Depositor

    Gerard Venema, Jan Kok

    Article

    van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    P32

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Pur-mEGFP

    Plasmid

    #186329

    Purpose

    Fluorescent reporter system

    Depositor

    Bertrand Collet

    Insert

    mEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX-EF1a_GFP_CdTag-HA

    Plasmid

    #221492

    Purpose

    GFP lentiviral overexpression of C-terminal dTag fusion

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-TALEN-L

    Plasmid

    #59025

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-L
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR1.cas9-NT

    Plasmid

    #197871

    Purpose

    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

    Depositor

    Baojun Wang

    Article

    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

    Inserts

    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAVS1-TALEN-R

    Plasmid

    #59026

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-R
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. TFORF0910

    Plasmid

    #142710

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BCLAF1 (BCLAF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-ZDHHC8

    Plasmid

    #231657

    Purpose

    Expresses human ZDHHC8 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC8 (ZDHHC8 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Flag-ZDHHC14

    Plasmid

    #231662

    Purpose

    Expresses human ZDHHC14 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC14 (ZDHHC14 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VN-Tau (P301L)

    Plasmid

    #87634

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    MAPT (MAPT Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Mutation P301L

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3.1-BCR-ABL-p210-FLAG

    Plasmid

    #205619

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p210 isoform b3a2
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

28. GFP-BRD7

    Plasmid

    #65379

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD7 (BRD7 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCSII-EF-miRFP670v1-hGem(1/110)

    Plasmid

    #80006

    Purpose

    hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hGeminin (1/110) (GMNN Human)
    

    Use

    Lentiviral

    Tags

    miRFP670v1

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUGW-APOE-E3-T2A-mCherry

    Plasmid

    #190808

    Purpose

    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry

    Depositor

    Ronald Hart

    Insert

    APOE (APOE Human)
    

    Use

    Lentiviral

    Mutation

    E3

    Promoter

    Ubi C

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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