169,450 results
-
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-mGFP-SNX9
Plasmid#223632PurposeLentiviral expression of human SNX9 with mGFP tag in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR
Plasmid#108104PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-BN
Plasmid#60518PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRPF3
Plasmid#155848PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK88_GFP-Anillin
Plasmid#46354DepositorInsertAnillin (ANLN Human)
UseRetroviralTagsGFPExpressionMammalianMutationa1854 is changed to c to disrupt XhoI site withou…PromoterpBABEAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-NLSmCherryNLS Clover-HDHB
Plasmid#190223PurposeFluorescent cell cycle reporterDepositorInsertsUseTransposonTagsClover fluorescent proteinExpressionMammalianMutationAmino Acids 994-1087PromoterEF1a and RPBSAAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9624-BEAR-cloning
Plasmid#162986PurposeBEAR target cloning plasmid with split EGFPDepositorInsertexchangeable cassette
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mouse Oct4-GFP GOF18 transgenic reporter
Plasmid#60527PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)DepositorInsertGFPpA
ExpressionMammalianPromoterOct4Available SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL119-TK
Plasmid#21911DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO mCherry (AAV Retrograde)
Viral Prep#114471-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Flp-dependent)Available SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-ER-TurboID
Plasmid#149412PurposeMuscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5PromotertMCKAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30D
Plasmid#214672PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice siteDepositorInsertMAPT (MAPT Human)
TagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30U
Plasmid#214673PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice siteDepositorInsertMAPT (MAPT Human)
TagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry
Plasmid#230043PurposePartial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.DepositorInsertlox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
UseCre/Lox; Zebrafish expressionPromoterfabp10aAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Coronin1B-N-10
Plasmid#54050PurposeLocalization: Cytoskeleton, Excitation: 487, Emission: 509DepositorAvailable SinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAT9752-BEAR-mScarlet
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK3b(Glu)
Plasmid#50449Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsFlagExpressionMammalianMutationchanged Serine 218 to Glutamic Acid, changed Thre…PromoterCMVAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAA)20-P2A-RFP
Plasmid#105688PurposeDouble fluorescent stalling reporter K(AAA)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-3xFLAG-NLS-GFP
Plasmid#198238PurposeLentivector with tet-inducible 3xFLAG-NLS-GFPDepositorInsert3xFLAG-NLS-GFP
UseLentiviralTags3xFLAGExpressionMammalianPromoterTREAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1
Plasmid#218817PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinityDepositorInsertiLACCO1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.1
Plasmid#218818PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinityDepositorInsertiLACCO1.1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iLACCO1.2
Plasmid#218819PurposeBacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinityDepositorInsertiLACCO1.2
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiLACCO1
Plasmid#218820PurposeBacterial expression of genetically encoded green fluorescent L-lactate control biosensorDepositorInsertDiLACCO1
ExpressionBacterialAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-23Q
Plasmid#84349PurposeExpression of the human Huntingtin Exon1 protein containing 23Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV9)
Viral Prep#26969-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEYFPAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI Intron c1
Plasmid#54433DepositorInsertIntron element for construction of silencing vectors (intron1 of AtWRKY33)
UseCloning vectorAvailable SinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only