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pBRG2

Plasmid

#149474

Purpose

Broad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

RhaS-RhaR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKG422 pBABE GFP-SPC24

Plasmid

#192696

Purpose

Retroviral transgene expression of human SPC24

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

SPC24 (SPC24 Human)

Use

Retroviral

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDD132

Plasmid

#119885

Purpose

Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

BbaJ23104_EYFP

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNLS-LEXY (pDN138)

Plasmid

#72657

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

NLS and mCherry-LEXY

Expression

Mammalian

Promoter

CMV-F

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-SEAP-26Sfur-3TM-tevs-KKMP

Plasmid

#179634

Purpose

TEVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Vector-Dendra2

Plasmid

#97101

Purpose

empty vector for overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Dis Model Mech. 2017 Sep 27. pii: dmm.03

Type

Empty backbone

Use

Zebrafish

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Article

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Promoter

hSYN

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-SO

Plasmid

#20329

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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PKC beta II DN

Plasmid

#16385

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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p006-GFP-weak

Plasmid

#108314

Purpose

GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

pFAB4282

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJF143.J3

Plasmid

#153028

Purpose

J3-mRFP1

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

J3-mRFP1

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pXWPrinA-gfp2

Plasmid

#123147

Purpose

pSB1K3 carrying PrinA-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

PrinA-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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