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pET28a-PTBP1-RRM12CCtoSS

Plasmid

#89155

Purpose

Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [2_n-1] (GB1206)

Plasmid

#75407

Purpose

tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [2_n-1]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pfast bac cry1myc

Plasmid

#51892

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

cry1 (Cry1 Mouse)

Tags

3x Flag and myc

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pfast bac Rbx-HA

Plasmid

#51893

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Rbx1 (RBX1 Human)

Tags

HA

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pHalo2X-mMYO7A-Full

Plasmid

#251425

Purpose

Mammalian expression vector for expressing HaloTag-fused full-length mouse MYO7A

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin VIIa (Myo7a Mouse)

Tags

HaloTag

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-HMM-FRB

Plasmid

#251424

Purpose

Mammalian expression vector for expressing a mouse MYO7A motor fragment with a C-terminal FRB

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin-VIIa Heavy Meromyosin (Myo7a Mouse)

Tags

FRB

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-mMYO7A-HMM-FKBP

Plasmid

#251423

Purpose

Mammalian expression vector for expressing a mouse MYO7A motor fragment with a C-terminal FKBP

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Insert

Myosin-VIIa Heavy Meromyosin (Myo7a Mouse)

Tags

FKBP

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

Plasmid

#229827

Purpose

A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xnls-TagBFP2-MCS-FLAG

Expression

Mammalian

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pscAAV.T7-SP6-BC(p11-14)-CAG-EGFP-SV40pA-T7

Plasmid

#231350

Purpose

Self complementary AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-mDLX-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231352

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter with mDLX enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-minBG-CI-mRuby2-W3SL-T7

Plasmid

#231353

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from minBG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-tdTomato-W3SL-T7

Plasmid

#231356

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses tdTomato from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CMVp-CI-mRuby2-W3SL-T7

Plasmid

#231357

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from CMV promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

Plasmid

#231358

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-SCP1-CI-mRuby2-W3SL-T7

Plasmid

#231359

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

Plasmid

#231348

Purpose

Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7-SP6-BC(p11-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC8

Plasmid

#224343

Purpose

Expresses human KDAC8 (HDAC8) in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Promoter

Polyhedrin

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-WPRE-bGH-pA (JDW 1221)

Plasmid

#224527

Purpose

A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-bGH

Use

Gateway cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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