We narrowed to 80,162 results for: ELL

1. pSCALPs-HA-NFAT1 (4-460)-GFP

   Plasmid

   #88879

   Purpose

   Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP fusion protein and HA tag

   Expression

   Mammalian

   Mutation

   amino acids 4-460 only; L78P & L287P *see bel…

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173202

   Purpose

   Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINK-OTUD6A (full-length, aa 1-288)

   Plasmid

   #61416

   Purpose

   Expresses human OTUD6A (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6A (OTUD6A Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3_HMOX1-V5-APEX2

   Plasmid

   #85823

   Purpose

   Expressed C-term tagged APEX2 on HMOX1 in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   HMOX1-V5-APEX2 (HMOX1 Human, Synthetic)
   

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Puro-TDP-43-A382T

   Plasmid

   #133756

   Purpose

   Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Mutation

   Changed alanine 382 to threonine

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-human LRRC33

   Plasmid

   #111602

   Purpose

   Express human LRRC33 in 293T cells

   Depositor

   Timothy Springer

   Article

   Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

   Insert

   human LRRC33 (NRROS Human)
   

   Tags

   myc/his tag

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1051 pBabe puroL Akt

   Plasmid

   #9011

   Depositor

   William Sellers

   Article

   DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pMXs-Lyl1

   Plasmid

   #131612

   Purpose

   expression of mouse LyI1 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Lyl1 (Lyl1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Expression

   Mammalian

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Tcf3

    Plasmid

    #131598

    Purpose

    expression of mouse Tcf3 in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Tcf3 (Tcf3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJP-CoV2leader-nLuc-TSS

    Plasmid

    #175430

    Purpose

    Expresses nLuc with 5' leader of CoV2 in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' CoV2 leader
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. RH5-bio

    Plasmid

    #47780

    Purpose

    Expresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised RH5
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJP-HBB-nLuc

    Plasmid

    #175431

    Purpose

    Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nLUC plus 5' HBB leader
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe WT Akt2

    Plasmid

    #14551

    Depositor

    Joan Brugge

    Article

    Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pH2R-mCherry-N1

    Plasmid

    #84326

    Purpose

    Histamine-2 receptor tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    H2R (HRH2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CSIV-TRE-ASCL1

    Plasmid

    #154883

    Purpose

    Doxycycline-dependently expresses human ASCL1.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPLINTR mCHERRY v1 backbone

    Plasmid

    #179778

    Purpose

    SPLINTR backbone vector

    Depositor

    Mark Dawson

    Article

    Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna3mciap1

    Plasmid

    #11474

    Depositor

    Jon Ashwell

    Article

    Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

    Insert

    mciap1 (Birc2 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only