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pLV hUbC-Cas9-T2A-GFP

Plasmid

#53190

Purpose

3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNoxa1

Plasmid

#58531

Purpose

expresses human Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (NOXA1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP DEST (736-1)

Plasmid

#19732

Purpose

3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCas9_GFP

Plasmid

#44719

Purpose

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCas9_GFP

Plasmid

#44719

Purpose

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pANAP

Plasmid

#48696

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

Depositor

Peter Schultz

Article

Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

Inserts

8 copies of the amber suppressor tRNACUA-Leu
AnapRS

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV and human H1 promoter

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-TAZ-N-Flag

Plasmid

#19025

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pEF-TAZ-N-Flag (S89A)

Plasmid

#19026

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin, S89A (site directed mu…

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pANAP

Plasmid

#48696

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

Depositor

Peter Schultz

Article

Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

Inserts

8 copies of the amber suppressor tRNACUA-Leu
AnapRS

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV and human H1 promoter

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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secreted BirA-Flag

Plasmid

#64395

Purpose

Flag-tagged biotinylation enzyme for co-expression with bio-proteins

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA (birA E. coli)

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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