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pET-11-LECT2-I40

Plasmid

#183032

Purpose

Expression of LECT2 I40 variant protein from T7 promoter

Depositor

Gareth Morgan

Article

Belonogov et al bioRxiv 2022.03.01.482540

Insert

LECT2 (LECT2 Human)

Expression

Bacterial

Mutation

I40 variant

Promoter

T7

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-eArch3.0-mRuby2-ST

Plasmid

#109135

Purpose

Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eArch3.0-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF3

Plasmid

#101336

Purpose

target site ATCAGATCTACAATGAAGGGC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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hMSH2 Clone E3 (full-length)

Plasmid

#16453

Depositor

Bert Vogelstein

Article

Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

Insert

MSH2 (MSH2 Human)

Expression

Bacterial

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pAG1237 FIREmate-ECFP-Fis1

Plasmid

#205173

Purpose

Expresses FIREmate-ECFP-Fis1 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Fis1

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LZF43: Cre-on CAG-CheRiff-CFP (DIO)

Plasmid

#104114

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTcI42-Wasabi

Plasmid

#86118

Purpose

mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TcI42
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A67T, K6N, S33T, Y61H, L119P, F122C

Promoter

pR/pL and pR/pL, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBA615

Plasmid

#186239

Purpose

eLbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-eLbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pAG1243 NES-ECFP-FIREmate

Plasmid

#205169

Purpose

Expresses NES-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NES-ECFP-FIREmate

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pQE80l ACA

Plasmid

#196471

Purpose

Protein expression of SpyTag-ELP-CarHC-ELP-SpyTag

Depositor

Fei Sun

Article

Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

Insert

ACA

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-EOS-GFP-IP

Plasmid

#60439

Purpose

PiggyBac for EOS-GFP

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Insert

EOS

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-ChETA-EYFP

Plasmid

#26967

Purpose

3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Insert

hChR2(E123T/H134R)-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

E123T

Promoter

CaMKIIa

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas9CyMutL

Plasmid

#166981

Purpose

Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

MutL (mutL )
CymR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

E32K

Promoter

pCymRC and pLacI

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Article

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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RET-WT

Plasmid

#125252

Purpose

Expresses human RET in mammalian cells

Depositor

Sissy Jhiang

Article

Xing et al Endocrinology. 1996 May;137(5):1512-9. d

Insert

RET (RET Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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