We narrowed to 10,688 results for: ada

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SpCas9 TadCBEc

Plasmid

#193834

Purpose

Express TadCBEc (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDc-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEb

Plasmid

#193832

Purpose

Express TadCBEb (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDb-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a Q

Plasmid

#89386

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pN565

Plasmid

#49990

Purpose

expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

T7 RNAP*

Use

Synthetic Biology

Tags

umuD degradation tag

Expression

Bacterial

Mutation

R632S

Promoter

pTac-symmetric

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-SH3(5R)

Plasmid

#112154

Purpose

Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3-5R (NCK1 Human)

Expression

Mammalian

Mutation

only the second SH3 domain is expressed in this c…

Promoter

CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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TIRAP-GFP

Plasmid

#52739

Purpose

Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pBAD_His B_QuasAr2

Plasmid

#64134

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2

Expression

Bacterial

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNA

Plasmid

#22661

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-UFD1L

Plasmid

#31560

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ubiquitin fusion degradation 1-like (UFD1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-N195K

Plasmid

#32708

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

N195K

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
PA-Dre

Plasmid

#169706

Purpose

Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag-T2A-pMag-Dre247

Use

AAV

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-L85R

Plasmid

#32707

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

L85R

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
AXBE

Plasmid

#204605

Purpose

Mammalian expression of adenine transversion base editor AXBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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