We narrowed to 10,808 results for: T7
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Plasmid#194089PurposeExpresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coliDepositorInsertEc86 effector-ncRNA-rrt
TagsHis8PromoterT7Available SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1531-DuET
Plasmid#213243PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AGTR1-DuET
Plasmid#213183PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet28b-His_RAD23A-UBA2
Plasmid#201550PurposeExpresses UBA2 of human RAD23A in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
Tags6x HisExpressionBacterialMutationExpresses amino acids 311-363 of DNA polymerase i…PromoterT7Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR + 5M→A_CR
Plasmid#194268PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with TDP-43 _CTD_5A→M_IDR + 5M→A_CRDepositorInsertTDP-43 (TARDBP Human, Synthetic)
Tags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M M322A, M323A, M…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-iLid
Plasmid#188456PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
Tags6xHis and iLidExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
Tags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4B, residues 156-224, no Cys
Plasmid#180641PurposeBacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140DepositorInsertCHMP4B (CHMP4B Human)
TagsHIS-SUMOExpressionBacterialMutationResidues 156-224PromoterT7Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TMSNKRS
Plasmid#198323PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPCNA-C148S
Plasmid#190939Purposeuntagged human PCNA with a cysteine to serine mutationDepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
ExpressionBacterialMutationchanged cysteine 148 to serinePromoterT7Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK_KRmutant-PTPN1
Plasmid#196447PurposeExpression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1DepositorExpressionBacterialMutationK591R, K639R, K702R, K865RPromoterT7 promoterAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas_4(I-U)/1_2_VB
Plasmid#186445PurposeEncodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducensDepositorInsertCas4/1 and Cas2
UseCRISPRMutationCas4 domain swapped with Cas4 domain from G. sulf…PromoterT7Available SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nmyc-Eps8L2_1-496-siR
Plasmid#196515PurposeExpression of Myc-Eps8L2_1-496DepositorInsertEps8L2_1-496 (EPS8L2 Human)
TagsMycExpressionMammalianMutationSilent mutations for siRNA resistancePromoterT7 promoterAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nmyc-Eps8L2-FL-siR
Plasmid#196514PurposeExpression of Myc-Eps8L2-FLDepositorInsertEps8L2-FL-siR (EPS8L2 Human)
TagsMycExpressionMammalianMutationSilent mutations for siRNA resistancePromoterT7 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE005
Plasmid#176762PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert101
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78_R41A
Plasmid#193368PurposeExpression of C9ORF78 R41A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78_R41A (C9orf78 Human)
TagsHis6-GST-TEVExpressionBacterialMutationR41A, the mutation is located in the binding inte…PromoterT7Available SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE070
Plasmid#176827PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert709
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE071
Plasmid#176828PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert710
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only